Skip to main content
SpringerLink
Log in

Characterization of the Brassica campestris mitochondrial gene for subunit six of NADH dehydrogenase: nad6 is present in the mitochondrion of a wide range of flowering plants

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Abstract

We have isolated the Brassica campestris mitochondrial gene nad6, coding for subunit six of NADH dehydrogenase. The deduced amino-acid sequence of this gene shows considerable similarity to mitochondrially encoded NAD6 proteins of other organisms as well as to NAD6 proteins coded for by plant chloroplast DNAs. The B. campestris nad6 gene appears to lack introns and produces and abundant transcript which is comparable in size to a previously described, unidentified transcript (#18) mapped to the B. campestris mitochondrial genome. An alignment of NAD6 proteins (deduced from DNA sequences) suggests that B. campestris nad6 transcripts are edited. Southern-blot hybridization indicates that nad6 is present in the mitochondrial genome of all of a wide range of flowering plant species examined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Attardi G, Schatz G (1988) Annu Rev Cell Biol 4:289–333

    Google Scholar 

  • Berger S, Ellersiek U, Steinmuller K (1991) FEBS Lett 286:129–132

    Google Scholar 

  • Binder S, Marchfelder A, Brennicke A, Wissinger B (1992) J Biol Chem 267:7615–7623

    Google Scholar 

  • Bland MM, Levings III CS, Matzinger DF (1986) Mol Gen Genet 204:8–16

    Google Scholar 

  • Boer PH, Gray MW (1989) Nucleic Acids Res 17:3993

    Google Scholar 

  • Brennicke A, Moller S, Blanz PA (1985) Mol Gen Genet 198:404–410

    Google Scholar 

  • Chapdelaine Y, Bonen L (1991) Cell 65:465–472

    Google Scholar 

  • Conklin PL, Wilson RK, Hanson MR (1991) Genes Dev 5:1407–1415

    Google Scholar 

  • Covello PS, Gray MW (1989) Nature 341:662–666

    Google Scholar 

  • Cummings DJ, Domenico JM (1988) J Mol Biol 204:815–839

    Google Scholar 

  • Cummings DJ, McNally KL, Domenico JM, Matsuura ET (1990) Curr Genet 17:375–402

    Google Scholar 

  • Dawson AJ, Jones VP, Leaver CJ (1984) EMBO J 3:2107–2113

    Google Scholar 

  • Devereux J, Haeberli P, Smithies O (1984) Nucleic Acids Res 12:387–395

    Google Scholar 

  • Desjardins P, Morais R (1990) J Mol Biol 212:599–634

    Google Scholar 

  • deVries S, Grivell LA (1988) Eur J Biochem 176:377–384

    Google Scholar 

  • Dupuis A, Skehel M, Walker JE (1991) Biochemistry 30:2954–2960

    Google Scholar 

  • Feagin JE, Stuart K (1985) Proc Natl Acad Sci USA 82:3380–3384

    Google Scholar 

  • Gass DA, Makaroff CA, Palmer JD (1992) Curr Genet 21:423–430

    Google Scholar 

  • Gruissem W, Barkan A, Deng XW, Stern D (1988) Trends Genet 4:258–263

    Google Scholar 

  • Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) Mol Gen Genet 215:118–127

    Google Scholar 

  • Gualberto J, Lamattina L, Bonnard G, Weil JH, Grienenberger JM (1989) Nature 341:660–662

    Google Scholar 

  • Gualberto JM, Weil JH, Grienenberger JM (1990) Nucleic Acids Res 18:3771–3776

    Google Scholar 

  • Hamby RK, Sims LE, Issel LE, Zimmer EA (1988) Plant Mol Biol Rep 6:175–192

    Google Scholar 

  • Hiesel R, Wissinger B, Schuster W, Brennicke A (1989) Science 246:1632–1634

    Google Scholar 

  • Jasmer DP, Feagin JE, Stuart K (1985) Mol Cell Biol 5:3041–3047

    Google Scholar 

  • Knoop V, Schuster W, Wissinger B, Brennicke A (1991) EMBO J 10:3483–3493

    Google Scholar 

  • Kolodner R, Tewari KK (1972) Proc Natl Acad Sci USA 69:1830–1834

    Google Scholar 

  • Koslowsky DJ, Bhat GJ, Perrollaz AL, Feagin JE, Stuart K (1990) Cell 62:901–911

    Google Scholar 

  • Lamattina L, Grienenberger JM (1991) Nucleic Acids Res 19:3275–3282

    Google Scholar 

  • Lonsdale DM (1989) The plant mitochondrial genome. In: Marcus A (ed) The biochemistry of plants, vol 15, Academic Press, New York, pp 230–295

    Google Scholar 

  • Macfarlane JL, Wahleithner JA, Wolstenholme DR (1990) Curr Genet 17:33–40

    Google Scholar 

  • Makaroff CA, Palmer JD (1987) Nucleic Acids Res 15:5141–5156

    Google Scholar 

  • Michaelis G, Vahrenholz C, Pratje E (1990) Mol Gen Genet 223:211–216

    Google Scholar 

  • Michel F, Umesono F, Ozeki H (1989) Gene 82:5–30

    Google Scholar 

  • Moller IM (1986) Physiol Plant 67:517–520

    Google Scholar 

  • Newton KJ (1988) Annu Rev Plant Physiol Plant Mol Biol 39:503–532

    Google Scholar 

  • Nugent JM, Palmer JD (1988) Curr Genet 14:501–509

    Google Scholar 

  • Nugent JM, Palmer JD (1991) Cell 66:473–481

    Google Scholar 

  • Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogur Y, Kohchi T, Ohyama K (1992) J Mol Biol 223:1–7

    Google Scholar 

  • Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota SI, Inokuchi H, Ozeki H (1986) Nature 322:572–574

    Google Scholar 

  • Payne M, Rothwell V, Jasmer DP, Feagin JE, Stuart K (1985) Mol Bio Parasitol 15:159–170

    Google Scholar 

  • Pritchard AE, Seilhamer JJ, Mahalingam R, Sable CL, Venuti SE, Cummings DJ (1990) Nucleic Acids Res 18:173–180

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schuster W, Hiesel R, Wissinger B, Brennicke A (1990a) Mol Cell Biol 10:2428–2431

    Google Scholar 

  • Schuster W, Wissinger B, Unseld M, Brennicke A (1990b) EMBO J 9:263–269

    Google Scholar 

  • Souza AE, Myler PJ, Stuart K (1992) Mol Cell Biol 12:2100–2107

    Google Scholar 

  • Wahleithner JA, Wolstenholme DR (1988) Nucleic Acids Res 16:6897–6913

    Google Scholar 

  • Wissinger B, Schuster W, Brennicke A (1991) Cell 65:473–482

    Google Scholar 

  • Xue Y, Davies DR, Thomas CM (1990) Mol Gen Genet 221:195–198

    Google Scholar 

Download references

Author information

Author notes

  1. Jacqueline M. Nugent

    Present address: John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

Authors and Affiliations

  1. Department of Biology, Indiana University, 47405, Bloomington, IN, USA

    Jacqueline M. Nugent & Jeffrey D. Palmer

Authors
  1. Jacqueline M. Nugent
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeffrey D. Palmer
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by D. R. Wolstenholme

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nugent, J.M., Palmer, J.D. Characterization of the Brassica campestris mitochondrial gene for subunit six of NADH dehydrogenase: nad6 is present in the mitochondrion of a wide range of flowering plants. Curr Genet 23, 148–153 (1993). https://doi.org/10.1007/BF00352014

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00352014

Key words

Search

Navigation