Abstract
In search for recombinants within the chicken major histocompatibility B complex, 1155 animals from crosses between the congenic lines CB (B12) and CC (B4) were tested with alloantibodies and monoclonal antibodies for the B-F (class I), B-L (class II), and B-G (class IV) antigens and by mixed lymphocyte reaction. The absence of detectable recombination was confirmed by restriction fragment length polymorphism analysis with B-L β and B-F probes. Together with previous reports, this indicates that the distance between the B-F and B-L loci is below 0.01 centimorgan.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benda, V., Vilhelmová, M., and Hála, K.: Stimulation of chicken spleen cell mitogenic response with turkey herpes virus vaccine against Marek's disease. Folia Biol (Praha) 24: 211–214, 1978
Bourlet, Y., Béhar, G., Guillemot, F., Fréchin, N., Billault, A., Chaussé, A. M., Zoorob, R., and Auffray, C.: Isolation of chicken major histocompatibility complex class II (B-L) β chain sequences: comparison with mammalian β chains and expression in lymphoid organs. EMBO J 7: 1031–1039, 1988
Briles, W. E., Bumstead, N., Ewert, D. L., Gilmour, D. G., Gogusev, J., Hála, K., Koch, C., Longenecker, B. M., Nordskog, A. W., Pink, J. R. L., Schierman, L. W., Simonsen, M., Toivanen, A., Vainio, O., and Wick, G.: Nomenclature for chicken major histocompatibility (B) complex. Immunogenetics 15: 441–447, 1982
Burg, R. W., Feldbush. T., Morris, C. A., and Maag, T. A.: Depression of thymus and bursa-dependent immune systems of chicks with Marek's disease. Avian Dis 15: 662–671, 1971
Crone, M. and Simonsen, M.: Avian major histocompatibility complex. In A. Toivanen and P. Toivanen (eds.): Avian Immunology: Basis and Practice, Volume II, p. 25–41, CRC Press, Boca Raton, 1987
Ewert, D. L., Gilmour, D. G., Briles, W. E., and Cooper, M. D.: Genetics of la-like alloantigens in chickens and linkage with B major histocompatibility complex. Immunogenetics 10: 169–174, 1950
Feinstein, A. and Vogelstein, B.: A technic for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1985
Guillemot, F., Billault, A., Pourquié, O., Béhar, G., Chaussé, A. M., Zoorob, R., Kreibich, G., and Auffray, C.: A molecular map of the chicken major histocompatibility complex: the class II β genes are closely linked to the class I genes and the nucleolar organizer. EMBO J, in press, 1988
Hála, K.: Inbred lines of Avian species. In A. Toivanen and P. Toivanen (eds.): Avian Immunology: Basis and Practice. Volume II, pp. 85–99, CRC Press, Boca Raton, 1987
Hála, K., Vilhelmová, M., and Hartmanová, J.: Probable crossing-over in the B blood group system of chickens. Immunogenetics 3: 97–103, 1976
Hála, K., Vilhelmová, M., Schulmanová, J., and Plachý, J.: New recombinant allele in the B-complex of the chicken. Folia Biol (Praha) 25: 323–324, 1979
Hála, K., Boyd, R., and Wick, G.: Chicken major histocompatibility complex and disease. Scand J Immunol 14: 607–617, 1981
Hartmanová, J., Hála, K., and Vilhelmová, M.: Differences in the distribution of chicken B complex antigens demonstrated by means of tolerance. Folia Biol (Praha) 23: 406–407, 1977
Koch, C., Skjoedt, K., Toivanen, A., and Toivanen, P.: New recom-binants within the MHC (B complex) of the chicken. Tissue Antigens 21: 129–137, 1983
Payne, L. N.: Immunosuppressive effect of avian oncogenic viruses. Proc Soc Med 63: 16–19, 1970
Pink, J. R., Dröge, W., Hála, K., Miggiano, V., and Ziegler, A.: A three-locus model for chicken major histocompatibility complex. Immunogenetics 5: 203–216, 1977
Schou, M.: A micromethod of chicken MLR: technical aspects and genetic control. Tissue Antigens 15: 373–380, 1980
Sgonc, R., Hála, K., and Wick, G.: Relationship between the expression of class I antigen and reactivity of chicken thymocytes. Immunogenetics 26: 150–154, 1987
Simonsen, M.: The MHC of the chicken, genomic structure, gene products and resistance to oncogenic DNA and RNA viruses. Vet Immunol Immunopathol 17: 243–253, 1987
Simonsen, M., Crone, M., Koch, C., and Hála, K.: The MHC haplotypes of the chicken. Immunogenetics 16: 513–532, 1982
Skjoedt, K., Koch, C., Crone, M., and Simonsen, M.: Analysis of chickens for recombination within the MHC (B-complex). Tissue Antigens 25: 278–282, 1985
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hála, K., Chaussé, AM., Bourlet, Y. et al. Attempt to detect recombination between B-F and B-L genes within the chicken B complex by serological typing, in vitro MLR, and RFLP analyses. Immunogenetics 28, 433–438 (1988). https://doi.org/10.1007/BF00355375
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00355375