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Modified 5′-termini in small nuclear RNAs of mouse myeloma cells

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Abstract

Nuclei of MPC 11 mouse myeloma cells contain several species of small RNAs related to those found in other mammalian cells. These include U1 RNA, about 190 nucleotides in length and U2 RNA, about 170 nucleotides long. The 5′-termini of 32P-labelled U1 and U2 RNAs have been investigated by a fingerprinting technique involving digestion with T2-ribonuclease. The RNAs were found to have modified 5′-terminal structures of the form m3 G(5′)ppp (5′)AmpUmpAp for U1 RNA and m3 G(5′)ppp(5′)AmpUmpCp for U2 RNA, where m3 G is N2, N27-trimethyl guanosine and Am and Um are 2′-O-methyl nucleosides. These 5′-terminal sequences are the same as those proposed for rat hepatoma U1 and U2 RNAs (Ro-Choi et al., Fed. Proc. 33, 1548, 1974) but with triphosphate rather than diphosphate links.

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Cory, S., Adams, J.M. Modified 5′-termini in small nuclear RNAs of mouse myeloma cells. Molecular Biology Reports 2, 287–294 (1975). https://doi.org/10.1007/BF00357015

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