Abstract
Nuclei of MPC 11 mouse myeloma cells contain several species of small RNAs related to those found in other mammalian cells. These include U1 RNA, about 190 nucleotides in length and U2 RNA, about 170 nucleotides long. The 5′-termini of 32P-labelled U1 and U2 RNAs have been investigated by a fingerprinting technique involving digestion with T2-ribonuclease. The RNAs were found to have modified 5′-terminal structures of the form m3 G(5′)ppp (5′)AmpUmpAp for U1 RNA and m3 G(5′)ppp(5′)AmpUmpCp for U2 RNA, where m3 G is N2, N27-trimethyl guanosine and Am and Um are 2′-O-methyl nucleosides. These 5′-terminal sequences are the same as those proposed for rat hepatoma U1 and U2 RNAs (Ro-Choi et al., Fed. Proc. 33, 1548, 1974) but with triphosphate rather than diphosphate links.
Similar content being viewed by others
References
Weinberg, R.A., Ann. Rev. Biochem. 42, 329–354 (1973).
Ro-Choi, T. S. and Busch, H., in H.Busch (ed.) The Molecular Biology of Cancer, pp 241–276, Academic Press, New York, (1974).
Ro-Choi, T. S., Reddy, R., Choi, Y. C. Raj, N. B., and Henning, D., Fed. Proc. 33, 1548 (1974).
Ro-Choi, T. S., Choi, Y. C., Henning, D., McCloskey, J., and Busch, H., J. Biol. Chem. 250, 3921 (1975).
Saponara, A. G. and Enger, M. D., Nature 223, 1365 (1969).
Furuichi, Y. and Miura, K., Nature 253, 374 (1975).
Wei, C. M. and Moss, B., Proc. Nat. Acad. Sci. U.S.A. 72, 318 (1975).
Urushibara, T., Furuichi, Y., Nishimura, C. and Miura, K-i, FEBS Letters 49, 385 (1975).
Furuichi, Y., Morgan, M., Muthukrishnan, S., and Shatkin, A. J., Proc. Nat. Acad. Sci. U.S.A. 72, 362 (1975).
Abraham, G., Rhodes, D. P., and Banerjee, A. K., Cell 5, 51 (1975).
Lavi, S. and Shatkin, A. J., Proc. Nat. Acad. Sci. U.S.A. in press (1975).
Adams, J. M. and Cory, S., Nature 255, 28 (1975).
Wei, C. M., Gershowitz, A., and Moss, B., Cell, in press (1975).
Furuichi, Y., Morgan, M., Shatkin, A. J., Jelinek, W., Salditt-Georgieff, M., and Darnell, J. E., Proc. Nat. Acad. Sci. U.S.A. 72, 1904 (1975).
Perry, R. P., Kelley, D. E., Friderici, K. and Rottman, F., Cell 4, 387 (1975).
Muthukishnan, S., Both, G. W., Furuichi, Y., and Shatkin, A. J., Nature 255, 33 (1975).
Laskov, R. and Scharff, M. D., J. Exp. Med. 131, 515 (1970).
Birckbichler, P. J. and Pryme, I. F., Eur. J. Biochem. 33, 368 (1973).
Blobel, G. and Potter, V. R., Proc. Nat. Acad. Sci. U.S.A. 55, 1283 (1966).
Rothe, J. S., Biochim. Biophys. Acta 21, 34 (1956).
Wiegers, U. and Hilz, H., Biochem. Biophys. Res. Comm. 44, 513 (1971).
Peacock, A. C. and Dingman, C. W., Biochemistry 6, 1818 (1967).
Ikemura, T. and Dahlberg, J. E., J. Biol. Chem. 248, 5024 (1973).
Barrell, B. G., Proc. Nucl. Acid. Res. 2, 751 (1971).
Sulkowski, E. and Laskowski, M., Biochim. Biophys. Acta 240, 443 (1971).
Duch, D. S., Borkowska, I., Stasiuk, L. and Laskowski, M., Anal. Biochem. 53, 459 (1973).
Rein, A. and Penman, S., Biochim. Biophys. Acta 190, 1 (1969).
Weinberg, R. A. and Penman, S., J. Mol. Biol. 38, 289 (1968).
Reddy, R., Ro-Choi, T. S., Henning, D. and Busch, H., J. Biol. Chem. 249, 6486 (1974).
Larsen, C. J., Lebowitz, P., Weissman, S. M., and DuBuy, B., Cold Spring Harbor Symp. Quant. Biol. 25, 35 (1970).
Shibata, H., Reddy, R., Henning, D., Ro-Choi, T. S., and Busch, H., Molecular and Cellular Biochemistry 1–16, in press (1975).
Klootwijk, J. and Planta, R. J., Eur. J. Biochem. 39, 325 (1973).
Razzell, W. E., Methods in Enzymol. 6, 236 (1963).
Randerath, K., Janeway, C. M., Stephenson, M. L., and Zamecnik, P. C., Biochim. Biophys. Res. Comm. 24, 98 (1966).
Nazar, R. N., Sitz, T. O., and Busch, H., Biochem. Biophys. Res. Comm. 62, 736 (1975).
Furuichi, Y., Nucleic. Acid. Res. 1, 809 (1974).
Faust, M. and Millward, S., Nucleic. Acid Res. 1, 1739 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cory, S., Adams, J.M. Modified 5′-termini in small nuclear RNAs of mouse myeloma cells. Molecular Biology Reports 2, 287–294 (1975). https://doi.org/10.1007/BF00357015
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00357015