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The chlorarachniophyte: a cell with two different nuclei and two different telomeres

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Abstract

Chlorarachniophyte algae contain a complex chloroplast derived from the endosymbiosis of a eukaryotic alga. The reduced nucleus of the endosymbiont, the nucleomorph, is located between the inner and outer pair of membranes surrounding the chloroplast. The nucleomorph of chlorarachniophytes has previously been demonstrated to contain at least three small linear chromosomes. Here we describe cloning the end of the smallest nucleomorph chromosome which is shown to carry a telomere consisting of a tandemly repeated 7 bp sequence, TCTAGGG. Using the telomere repeat as a probe, we show that nucleomorph telomeres display typical hetero-disperse size distribution. The nucleomorph is shown to contain only three chromosomes with a haploid genome size of just 380kb. All six nucleomorph chromosome termini are identical with an rRNA cistron closely linked to the telomere. The nucleomorph chromosomes thus have relatively large inverted repeats at their ends. Chromosomes from the host nucleus are shown to have a different telomere repeat motif to that of the nucleomorph chromosomes.

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References

  • Bendich AJ (1993) Reaching for the ring: the study of mitochondrial genome structure. Curr Genet 24: 279–290

    Google Scholar 

  • Biessmann H, Mason JM (1994) Telomeric repeat sequences. Chromosoma 103: 154–161

    Google Scholar 

  • Boynton JE, Gillham NW, Newman SM, Harris EH (1992) Organelle genetics and transformation of Chlamydomonas. In: Hermann HG (ed) Plant gene research Cell organelles. Springer-Verlag, Wien, New York, pp 31–34

    Google Scholar 

  • Charron MJ, Read E, Haut SR,Michels CA (1989) Molecular evolution of the telomere-associated MAL Loci of Sacharomyces. Genetics 122: 307–316

    Google Scholar 

  • De Bruin D, Lanzer M, Ravetch JV (1994) The polymorphic subtelomeric regions of Plasmodium falciparum chromosomes contain arrays of repetitive sequence elements. Proc Natl Acad Sci USA 91: 619–623

    Google Scholar 

  • Eschbach S, Hofmann CJB, Maier UG, Sitte P, Hansmann P (1991) A eukaryotic genome of 660 kb: electrophoretic karyotype of neucleomorph and cell nucleus of the cryptomonad alga. Pyrenomonas salina. Nucleic Acids Res 19: 1779–1781

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragements to high specific activity. Anal Biochem 132: 6–13

    Google Scholar 

  • Greider CW, Autexier C, Avilion AA, Collins K, Harrington LA, Mantell LL, Prowse KR, Smith SK, Allsop RC, Counter CM, Vaziri H, Bacchetti S, Harley CB (1993) Terlomeres and telomerase: biochemistry and regulation in senescence and immortalization. In: Heslop-Harrison JS, Flavell RB (eds) The chromosome. Blos Scientific Publishers, Oxford, UK, pp 115–125

    Google Scholar 

  • Hansmann P, Eschbach S (1990) Isolation and preliminary characterisation of the nucleus and the nucleomorph of a cryptomonad, Pyrenomonas salina. Eur J Cell Biol 52: 373–378

    Google Scholar 

  • Howe CJ, Beanland TJ, Larkum AWD, Lockhart PJ (1992) Plastid origins. Trends Ecol Evol 7: 378–383

    Google Scholar 

  • Le Blancq SM, Kase RS, Van der Ploeg LHT (1991) Analysis of a Giardia lamblia rRNA encoding telomere with [TAGGG]n as the telomere repeat. Nucleic Acids Res 19: 5790

    Google Scholar 

  • McFadden GI, Gilson PR, Hofmann CJB, Adcock GJ, Maier UG (1994a) Evidence that an amoeba acquired a chloroplast by retaining part of an engulfed eukaryotic alga. Proc Natl Acad Sci USA 91: 3690–3694

    Google Scholar 

  • McFadden GI, Gilson PR, Douglas SE (1994b) The photosynthetic endosymbiont in cryptomonad cells produces both chloroplast and cytoplasmic-type ribosomes. J Cell Sci 107: 649–657

    Google Scholar 

  • Ohyama K (1992) Organization and expression of genes of plastid chromosomes from non-angiospermous land plants and green algae In: Hermann HG (ed) Plant gene research. Cell organelles. Springer-Verlag, Wien, New York, pp 137–163

    Google Scholar 

  • Palmer JD (1992) Comparison of chloroplasts and mitochondrial genome evolution in plants. In: Hermann HG (ed) Plant gene research Cell organelles. Springer-Verlag, Wien, New York, pp 99–133

    Google Scholar 

  • Powell WA, Kistler HC (1990) In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids. J Bacteriol 1990: 3163–3171

    Google Scholar 

  • Reith M, Munholland J (1993) A high-resolution gene map of the chloroplast genome of the red alga Porphyra purpurea. Plant Cell 5: 465–475

    Google Scholar 

  • Richards EJ, Ausubel FM (1988) Isolation of a higher eurkaryotic telomere from Arabidopsis thaliana. Cell 53: 127–136

    Google Scholar 

  • Richards EJ, Vongs A, Wlash M, Yang J, Chao S (1993) Substructure of telomere repeat arrays. In: Heslop-Harrison JS, Flavell RB (eds) The chromosome. Bios Scientific Publishers. Oxford, UK, pp 103–114

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Schechtman MG (1990) Characterisation of telomere DNA from Neurospora crassa. Gene 88: 159–165

    Google Scholar 

  • Shampay J, Szostack JW, Blackburn EH, (1984) DNA sequences of telomeres maintained in veast. Nature 310: 154–157

    Google Scholar 

  • Shippen DE (1993) Telomeres and telomerases. Curr Opin Genet Dev 3: 759–763

    Google Scholar 

  • Vahrenholz C, Riemen G, Pratje E, Dujon B, Michaelis G (1993) Mitochondrial DNA of Chlamydomonas reinhardtii the structure of the ends of the 15.8-kb genome suggests mechanisms for DNA replication. Curr Genet 24: 241–247

    Google Scholar 

  • Wu K-S, Tanksley SD (1993) Genetic and physical mapping of telomeres and macrosatellites of rice. Plant Mol Biol 22: 861–872

    Google Scholar 

  • Zakian VA (1989) Structure and function of telomeres. Annu Rev Genet 23: 579–604

    Google Scholar 

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Gilson, P., McFadden, G.I. The chlorarachniophyte: a cell with two different nuclei and two different telomeres. Chromosoma 103, 635–641 (1995). https://doi.org/10.1007/BF00357690

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