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Effects of hydration and dehydration on cyclic adenosine 3′,5′-monophosphate concentration in the rat kidney

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Summary

Renal 3′,5′-AMP concentration has been measured in two groups of rats, one of which was hydrated, the other one was dehydrated by withdrawal of drinking-water for a period of 4–5 days. The first group was assumed to have low, the latter to have high vasopressin (ADH) plasma concentrations.

The 3′,5′-AMP concentration in the kidney of hydrated rats was 1.7 nmoles/g (w.w.). Studies on the distribution of 3′,5′-AMP between various regions of the kidney revealed that the 3′,5′-AMP content of the cortex was higher than in the outer medulla. 3′,5′-AMP concentration in the inner medulla was below the sensitivity of the method used for the determination of the nucleotide.

Dehydration led to a doubling of renal 3′,5′-AMP concentration. The increment in the cortex was greater than in the outer medulla. In the inner medulla 3′,5′-AMP concentration remained below the sensitivity of the method used.

Renal 3′,5′-AMP phosphodiesterase has been obtained from two subcellular fractions, one fraction bound to large particles, probably cell membranes, the other one soluble in the supernatant. Vasopressin did alter the activity of the enzyme in neither fractions. From this it has been concluded that the increase in 3′,5′-AMP concentration in the kidney of dehydrated rats results from stimulation of adenyl cyclase by ADH.

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Authors and Affiliations

  1. Department of Pharmacology, Freie Universität Berlin, Germany

    G. Senft, M. Hoffmann, K. Munske & G. Schultz

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  1. G. Senft
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  2. M. Hoffmann
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  3. K. Munske
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  4. G. Schultz
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Additional information

This study was supported by the Deutsche Forschungsgemeinschaft.

Deceased October 31, 1967.

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Senft, G., Hoffmann, M., Munske, K. et al. Effects of hydration and dehydration on cyclic adenosine 3′,5′-monophosphate concentration in the rat kidney. Pflügers Archiv 298, 348–358 (1968). https://doi.org/10.1007/BF00363874

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  • DOI: https://doi.org/10.1007/BF00363874

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