Abstract
The chromosomal location of the human V pre B gene was determined by Southern blotting analysis of restriction enzyme-digested DNAs from a panel of 17 mouse-human somatic cell hybrids. The pattern of hybridization of a VpreB-specific probe in conjunction with earlier analysis of several marker genes allowed the following conclusions: 1) V pre B is on human chromosome 22 within band 22q11.2 distal to the bcr-like gene, bcr-2 and proximal to the bcr-like gene, bcr-4. 2) VpreB has been localized relative to several constitutional and tumor-specific breakpoints within 22q 11.2, segregates in hybrids retaining 22q−chromosomes with some but not with all members of the \({\text{V}}_{\lambda _1 } \) subgroup of the V λgenes, and is amplified with these genes in K562 cells. 3) The order of the loci on chromosome 22 is centromere→bcr-2, V preB, \({\text{V}}_{\lambda _1 } \to bcr - 4 \to C_\lambda \to bcr - 1 \to bcr - 3 \to sis\).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bauer, S. R., Kudo, A., and Melchers, F.: Structure and pre-B lymphocyte restricted expression of the VpreB gene in humans and conservation of its structure in other mammalian species. EMBO J 7: 111–116, 1988
Bertrand, S., Berger, R., Philip, T., Bernheim, A., Bryon, P. A., Bertoglio, J., Dore, J. F., Brunat-Mentigny, M., and Lenoir, G. M.: Variant translocation in a nonendemic case of Burkitt's lymphoma t (8; 22) in an Epstein-Barr virus negative tumor and in a derived cell-line. Eur J Cancer Clin Oncol 17: 577–584, 1981
Budarf, M., Sellinger, B., Griffin, C., and Emanuel, B. S.: Molecular mapping of the constitutional and tumor associated 11: 22 translocation. Am J Hum Genet 41: A114, 1987
Cannizzaro, L. A., Croce, C. M., Griffin, C. A., Simeone, A., Boncinelli, E., and Huebner, K.: Human homeo box-containing genes located at chromosome regions 2g31-2837 and 12g12→12g13. Am J Hum Genet 41: 1–15, 1987
Croce, C. M., Thierfelder, W., Erikson, J., Nishikura, K., Finan, J., Lenoir, G. M., and Nowell, P. C.: Transcriptional activation of unrearranged and untranslocated c-myc locus in Burkitt lymphoma cells. Proc Natl Acad Sci USA 80: 6922–6926, 1983
Croce, C. M., Huebner, K., Isobe, M., Fainstein, E., Lifshitz, B., Shtivelman, E., and Canaani, E.: Mapping of four distinct BCR-related loci to chromosome region 22g11: order of BCR loci relative to chronic myelogenous leukemia and acute lymphoblastic leukemia breakpoints. Proc Natl Acad Sci USA 84: 7174–7178, 1987
Dalla-Favera, R., Bregni, M., Erikson, J., Patterson, D., Gallo, R. C., and Croce, C. M.: Human c-myc one gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc Natl Acad Sci USA 79: 7824–7827, 1982a
Dalla-Favera, R., Gallo, R. C., Giallongo, A., and Croce, C. M.: Chromosomal localization of the human homology (c-sis) of the simian sarcoma virus one gene. Science 218: 686–688, 1982b
d'Eustachio, P., Bothwell, A. L. M., Takaro, T. K., Baltimore, D., and Ruddle, F. H.: Chromosomal location of structural genes encoding murine immunoglobulin A light chain. Genetics of murine λ light chains. J Exp Med 153: 793–800, 1981
Durst, M., Croce, C. M., Gissmann, L., Schwarz, E., and Huebner, K.: Papillomavirus sequences integrate near cellular oncogenes in some cervical carcinomas. Proc Natl Acad Sci USA 84: 1070–1074, 1987
Emanuel, B. S., Nowell, P. C., McKeon, C., Croce, C. M., and Israel, M.: Translocation breakpoint mapping: molecular and cytogenic studies of chromosome 22. Cancer Genet Cytogenet 19: 81–92, 1986
Erikson, J., Griffin, C. A., ar-Rushdi, A., Valtieri, M., Hoxie, J., Finan, J., Emanuel, B. S., Rovera, G., Nowell, P. C., and Croce, C. M.: Heterogeneity of chromosome breakpoint in Philadelphia positive (Ph+) acute lymphocytic leukemia. Proc Natl Acad Sci USA 83: 1807–1811, 1986
Feinberg, A. P. and Vogelstein, B.: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267, 1984
Hieter, P. A., Hollis, G. F., Korsmeyer, S. J., Walmann, T. A., and Leder, P.: Clustered arrangement of inimunoglobulin λ constant region genes in man. Nature 294: 536–540, 1981
Huebner, K., Isobe, M., Croce, C. M., Golde, D. W., Kaufman, S. E., and Gasson, J. C.: The human gene encoding GM-CSF is at 5g21-q32, the chromosome region deleted in the 5q− anomaly. Science 230: 1282–1285, 1985
Huebner, K., Isobe, M., Gasson, G. C., Golde, D. W., and Croce, C. M.: Localization of the gene encoding human erythroid potentiating activity to chromosome region Xp 11.1→Xp 11.4. Am J Hum Genet 38: 819–826, 1986
Kozbor, D., Giallongo, A., Sierzega, M. E., Konopka, J. B., Witte, O. N., Showe, L. C., and Croce, C. M.: Expression of a translocated c-abl gene in hybrids between mouse fibroblasts and chronic meylogenous leukemia cells. Nature 319: 331–333, 1986
Kudo, A. and Melchers, F.: A second gene, VpreB in the λ5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes. EMBO J 6: 2267–2272, 1987
Kudo, A., Sakaguchi, N., and Melchers, F.: Organization of the murine Ig-related λ 5 gene transcribed selectively in pre-B lymphocytes. EMBO J 6: 103–107, 1987a
Kudo, A., Pravtcheva, D., Sakaguchi, N., Ruddle, F. H., and Melchers, F.: Localization of the murine λ5 gene on chromosome 16. Genomics 1: 277–279, 19876
Lange, B., Valtieri, M., Santoli, D., Caracciolo, D., Mavilio, F., Gemperlein, I., Griffin, C., Emanuel, B., Finan, J., Nowell, P., and Rovera, G.: Growth factor requirements of childhood acute leukemia: establishment of GM-CSF dependent cell lines. Blood 70: 192–199, 1987
Lifshitz, B., Fainstein; E., Marcelle, C., Shtivelman, E., Amson, R., Gale, R. P., and Canaani, E.: bcr genes and transcripts. Oncogene 2: 113–117, 1988
Lozzio, C. and Lozzio, B. B.: Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321–334, 1975
Magrath, I., Erikson, J., Whang-Peng, J., Sieverts, H., Armstrong, G., Benjamin, D., Triche, T., Alabaster, O., and Croce, C. M.: Synthesis of Kappa light chains by cell-lines containing an 8; 22 chro mosomal translocation derived from a male homosexual with Burkitt's lymphoma. Science 222: 1094–1098, 1983
McBride, D. W., Hieter, P. A., Hollis, G. F., Swan, D., Otey, M. C., and Leder, P.: Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes. J Exp Med 155: 1480–1490, 1982
Nowell, P., Shankey, T. V., Finan, J., Guerry, D., and Besa, E.: Proliferation, differentiation, and cytogenetics of chronic leukemic B lymphocytes cultured with mitomycin-treated normal cells. Blood 57: 444–451, 1981
Nowell, P. C., Vonderheid, E. C., Besa, E., Hoxie, J. A., Moreau, L., and Finan, J. B.: The most common chromosomal change in 86 chronic B cell or T cell tumors: a 14q32 translocation. Cancer Genet Cytogenet 19: 219–227, 1986
Reed, K. C. and Mann, D. A.: Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13: 7207–7221, 1985
Sakaguchi, N. and Melchers, F.: λ5, a new light chain-related locus selectively expressed in pre B lymphocytes. Nature 324: 579–582, 1986
Sakaguchi, N., Berger, C. N., and Melchers, F.: Isolation of a cDNA copy of an RNA species expressed in murine pre B cells. EMBO J 5: 2139–2147, 1986
Selden, J. R., Emanuel, B. S., Wang, E., Cannizzaro, L., Palumbo, A., Erikson, J., Nowell, P. C., Rovera, G., and Croce, C. M., Amplified cλ and c-abl genes are on the same marker chromosome in K562 leukemic cells. Proc Natl Acad Sci USA 80: 7289–7292, 1983
Showe, L. C., Moore, R. C. A., Erikson, J., and Croce, C. M.: MYC oncogene involved in a t(8; 22) chromosome translocation is not altered in its putative regulatory region. Proc Natl Acad Sci USA 84: 2824–2828, 1987
Sun, L. K., Croce, C. M., and Showe, L. C.: Cloning and sequencing of a rearranged V λ gene from a Burkitt's lymphoma cell line expressing kappa light chain. Nucleic Acids Res 13: 4921–4934, 1985
Tsujimoto, Y. and Croce, C. M.: Molecular cloning of a human immunoglobulin λ chain variable sequence. Nucleic Acids Res 12: 8407–8414, 1984
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bauer, S.R., Huebner, K., Budarf, M. et al. The human V pre B gene is located on chromosome 22 near a cluster of \({\text{V}}_{\lambda _1 } \) gene segments. Immunogenetics 28, 328–333 (1988). https://doi.org/10.1007/BF00364231
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00364231