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The role of phenylacetic acid in biosynthesis of penicillin amidase in E. coli

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Abstract

For improved biosynthesis of penicillin amidase by E. coli, NCIM 2400, the participation of different carboxylic acids and polyols has been studied in association with the effect of phenylacetic acid. A modified defined medium has been devised for this purpose.

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References

  1. Babu, P. S. R.; Panda, T.: Cellular control mechanism for the biosynthesis of penicillin amidase in E. coli. In: National Institute of Rural Development, Proc. Seminar on “Application of Biotechnology for Agriculture and Rural Development”, pp. 116–135. Hyderabad (India), 1988

  2. Babu, P. S. R.; Panda, T.: Chemo- and stereo-specificity in penicillin acylase. In: Proc. 41st Annual session of Indian Institute of Chemical Engineers: I. C-4, p. 19, MS University, Baroda (India), 1988

    Google Scholar 

  3. Babu, P. S. R.; Panda, T.: Effect of aeration on the biosynthesis of penicillin amidase in E. coli. In: Proc. CHEMCON '89 (42nd Annual session of Indian Institute of Chemical Engineers); Trivandrum (India) II (1989) 507–510

  4. Klein, J.; Wagner, F.: Immobilization of whole microbial cells for the production of 6-APA. Enzyme Engg. 5 (1980) 335–345

    Google Scholar 

  5. Levitor, M. M.; Kla Povskiya, K. I.; Kleiner, G. I.: The induced acylase biosynthesis of E. coli. Mikrobiologiya 36 (1967) 912–917

    Google Scholar 

  6. Vojtisek, V.; Slezak, J.: Penicillin amidohydrolase in E. coli. Folia Microbiologia 20 (1975) 289–306

    Google Scholar 

  7. Mallette, M. F.: Evaluation of growth by physical and chemical methods. In: Norris, J. R.; Ribbons, D. W. (Eds.): Methods in Microbiology, vol. 1, pp. 521–566. New York: Academic Press 1971

    Google Scholar 

  8. Balasingham, K.; Worburton, P.; Dunnill, P.; Lilly, M. D.: The isolation and kinetics of penicillin amidase from E. coli: Biochim. Biophys. Acta 276 (1972) 250–256

    Google Scholar 

  9. Manual of Elemental Analyzer (Carlo Erba Strumentazione S.P.A. Milan)

  10. Elsworth, R.; Miller, G. A.; Whitaker, A. R.; Kitching, D.; Sayer, P. D.: Production of Escherichia coli as a source of nucleic acids. J. Appl. Chem. 17 (1968) 157–166

    Google Scholar 

  11. Verhoff, F. H.; Boguslawski, G.; Lantero, O. J.; Schlager, S. T.: Glucose isomerase: In: Moo-Young, M. (Ed.): Comprehensive Biotechnology, vol. 3, pp. 837–859. New York: Pergamon Press 1985

    Google Scholar 

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Authors and Affiliations

  1. Division of Biochemical Engineering Department of Chemical Engineering, Indian Institute of Technology, 600 036, Madras, India

    P. S. R. Babu & T. Panda

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  1. P. S. R. Babu
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  2. T. Panda
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Babu, P.S.R., Panda, T. The role of phenylacetic acid in biosynthesis of penicillin amidase in E. coli. Bioprocess Eng. 6, 71–74 (1990). https://doi.org/10.1007/BF00369280

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  • DOI: https://doi.org/10.1007/BF00369280

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