Abstract
Cells of Clostridium acetobutylicum were immobilized by adsorption onto bonechar. and used for the production of solvents (ABE fermentation) from whey permeate. When the process was performed in packed bed reactors operated in a vertical or inclined mode, solvent productivities approximating 6 kg/(m3h) were obtained. However, the systems suffered from blockage due to excess biomass production and gas hold-up. These problems were less apparent when a partially-packed bed reactor was operated in the horizontal mode. A fluidized bed reactor proved to be the most stable of the systems investigated, and a productivity of 4.8 kg/(m3h) was maintained over a period of 2000 h of operation. The results demonstrate that this type of reactor may have a useful future role in the ABE fermentation.
Similar content being viewed by others
References
Toda, K.; Ohtake, H.; Asakura, T.: Ethanol production in horizontal bioreactor. Appl. Microbiol. Biotechnol. 24 (1986) 97–101
Shiotani, T.; Yamane, T.: A horizontal packed bed bioreactor to reduce CO2 holdup in the continuous production of ethanol by immobilized yeast cells. Eur. J. Appl. Microbiol. Biotechnol. 13 (1981) 96–101
Juneja, L. R.; Terasawa, M.; Yamane, T.; Shimizu, S.: Continuous ethanol fermentation using flocculent yeast entrapped in horizontal parallel flow bioreactor system. Biotechnol. Lett. 8 (1986) 431–436
Chotani, G. K.; Constantinides, A.: Immobilized cell cross flow reactor. Biotechnol. Bioeng. 26 (1984) 217–220
Krouwel, P. G.; Groot, W. J.; Kossen, N. W. F.; van der Laan, W. F. M.: Continuous isopropanol butanol and ethanol fermentation by immobilized Clostridium beijerinckii cells in a packed bed fermenter. Enzyme Microb. Technol. 5 (1983) 46–54
Ennis, B. M.; Maddox, I. S.; Schoutens, G. H.: Immobilized Clostridium acetobutylicum for continuous butanol production from whey permeate. New Zealand J. Dairy Sci. Technol. 21 (1986) 99–109
Qureshi, N.; Maddox, I. S.: Continuous solvent production from whey permeate using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Enzyme Microb. Technol. In press
Qureshi, N.; Pai, J. S.; Tamhane, D. V.: Reactors for ethanol production using immobilized yeast cells. J. Chem. Technol. Biotechnol. 39 (1987) 75–84
Ennis, B. M.; Maddox, I. S.: Use of Clostridium acetobutylicum P262 for production of solvents from whey permeate. Biotechnol. Lett. 7 (1985) 601–606
Ennis, B. M.; Qureshi, N.; Maddox, I. S.: In-line toxic product removal during solvent production by continuous fermentation using immobilized Clostridium acetobutylicum. Enzyme Microb. Technol. In press
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Qureshi, N., Maddox, I.S. Reactor design for the ABE fermentation using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Bioprocess Eng. 3, 69–72 (1988). https://doi.org/10.1007/BF00369330
Issue Date:
DOI: https://doi.org/10.1007/BF00369330