Abstract
As a part of the investigations on the microbial lipid production using the yeast Rhodotorula gracilis, CFR-1, kinetics of the biomass synthesis has been studied using shake flask experiments. Using a medium containing a carbon to nitrogen ratio of 70∶1, the rates of biomass production were followed at different initial substrate concentrations in the range of 20–100 kg/m3. A logistic model was found to be reasonably adequate to describe the kinetics of the growth of biomass; the maximum specific growth rate of 0.105 h−1 was applicable for substrate concentrations less than 60 kg/m3, which gave reasonable agreement between predicted and actual biomass concentration values.
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Abbreviations
- S 0, X 0 kg/m3 :
-
Initial concentrations of sugar, non lipid biomass respectively
- X, X(t) kg/m3 :
-
Concentrations of non lipid biomass at any time t
- dX/dt kg/(m3 · h):
-
Rate of biomass growth
- μ h−1 :
-
Specific growth rate
- μ max h−1 :
-
Maximum specific growth rate
- K s mol/dm3 :
-
Monods constant
- X max kg/m3 :
-
Maximum biomass reached in a run
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Karanth, N.G., Sattur, A.P. Mathematical modeling of production of microbial lipids. Bioprocess Engineering 6, 227–234 (1991). https://doi.org/10.1007/BF00369716
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DOI: https://doi.org/10.1007/BF00369716