Abstract
A monoclonal antibody defining a unique mouse neutrophil cell-surface antigen, Gm-3.2, is described. Gm-3.2 is found on all neutrophils in peritoneal exudates and in bone marrow, and is also present on macrophages activated by thioglycolate but is absent from lymphoid, kidney, liver, heart, and red cells. Gm-3.2 is a differentiation antigen of myeloid cells, as granulocyte/macrophage colony-forming cells are Gm-3.2− while mature neutrophils are Gm-3.2+. Strain distribution pattern analysis shows linkage of the Gm-3 locus to the Ly-4, B2m, H-3 complex on chromosome 2.
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Austyn, J. M. and Gordon, S.: F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805–815, 1981
Davidson, W. F. and Parish, C. R.: Procedures for removing red cells and dead cells from lymphoid cell suspensions. J. Immunol. Methods 7: 291–300, 1975
Harris, R. A., Hogarth, P. M., McKenzie, I. F. C., and Penington, D. S.: Differential expression of Qa-m2 alloantigen on murine hemopoietic progenitor cells. Exp. Hematol. 11: 527–541, 1983
Hibbs, M. L., Hogarth, P. M., Scott, B. M., Harris, R. A., and McKenzie, I. F. C.: Monoclonal antibody to murine neutrophils: Identification of the Gm-2.2 specificity. J. Immunol. 133: 2619–2623, 1984
Hirsch, S. and Gordon, S.: Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4. Immunogenetics 18: 229–239, 1983
Hogarth, P. M. and McKenzie, I. F. C.: Lymphocyte antigens. In W. E. Paul (ed.): Fundamental Immunology, pp. 457–477, Raven Press, New York, 1983
LeBlanc, P. A., Katz, H. R., and Russell, S. W.: A discrete population of mononuclear phagocytes detected by monoclonal antibody. J. Infect. Immun. 29: 520–525, 1980
Lin, H. S. and Gordon, S.: Secretion of plasminogen activator by bone marrow-derived mononuclear phagocytes and its enhancement by colony-stimulating factor. J. Exp. Med. 150: 231–245, 1979
McKenzie, I. F. C. and Snell, G. D.: Ly-4.2: A cell membrane alloantigen of murine B lymphocytes. I. Population studies. J. Immunol. 114: 848–855, 1975
Michaelson, J.: Genetic polymorphism of β2-microglobulin (B2m) maps to the H-3 region of chromosome 2. Immunogenetics 13: 167–171, 1981
Parish, C. R. and McKenzie, I. F. C.: A sensitive rosetting method for detecting subpopulations of lymphocytes which react with alloantisera. J. Immunol. Methods 20: 173–183, 1978
Parish, C. R., Kirov, S. M., Bowern, N., and Blanden, R. V.: A one step procedure for separating mouse T and B lymphocytes. Eur. J. Immunol. 4: 808–815, 1974
Potter, T. A., Watt, S. M., Burgess, A. W., and McKenzie, I. F. C.: Characterization of surface alloantigens of murine neutrophils. Immunogenetics 8: 461–473, 1979
Potter, T. A., McKenzie, I. F. C., and Cherry, M. C.: Murine lymphocyte alloantigens: The Ly-4 locus. J. Immunogenet. 8: 15–25, 1981
Sandrin, M. S., Potter, T. A., Morgan, G. M., and McKenzie, I. F. C.: Detection of mouse alloantibodies by rosetting with protein-A coated sheep red blood cells. Transplantation 26: 126–130, 1978
Springer, T. A.: Mac-1,2,3 and 4; murine macrophage differentiation antigens identified by monoclonal antibodies. In O. Förster and M. Landy (eds.): Heterogenity of Mononuclear Phagocytes, p. 37, Academic Press, New York, 1981
Watt, S. M., Burgess, A. W., and Metcalf, D.: Isolation and surface labelling of murine polymorphonuclear neutrophils. J. Cell Physiol. 100: 1–22, 1979
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Hibbs, M.L., Hogarth, P.M., Harris, R.A. et al. Gm-3.2, A new granulocyte/macrophage alloantigen. Immunogenetics 21, 61–70 (1985). https://doi.org/10.1007/BF00372242
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DOI: https://doi.org/10.1007/BF00372242