Skip to main content
SpringerLink
Log in

Development of a new method for testing strength of cells against fluid shear stress

  • Originals
  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

A new method for testing the strength of cells against fluid shear stress by using a long capillary column was proposed. The trajectories of cells in the column were simulated by introducing the Brownian motion model. The Brownian motion was performed by the generation of random numbers. The mean exposure time to shear stress and the mean shear stress acting on the surface of cells were discussed by the result of computer simulation. The mean shear stress acting on the surface of cells flowing in the capillary column was estimated as 4/3-fold of the shear stress at the column wall provided that the ratio of the cell radius to the column radius does not exceed 0.08. The effectiveness of this new method for testing the strength of cells against fluid shear stress was shown.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

a m:

radius of cell

c :

constant

E :

distribution function

L m:

length of capillary column

M :

number of division

N :

number of division

p :

probability

Q m3/s:

flow rate

R m:

radius of capillary column

r m:

radial position

t s:

time

T s:

exposure time

T m s:

mean exposure time

T 0 s:

mean residence time

μ m/s:

axial velocity

u m m/s:

cross-sectional flow velocity

z m:

axial position

γ s−1 :

shear rate

γ w s−1 :

shear rate at wall

μ Pa s:

viscosity

θ :

spherical coordinate

φ :

spherical coordinate

τ Pa:

shear stress

τ m Pa:

mean shear stress

τ w Pa:

shear stress at wall

References

  1. Bussolari, S. R.; Dewey Jr., C. F.; Gimbrone Jr., M. A.: Apparatus for subjecting living cells to fluid shear stress. Rev. Sci. Instrum. 53 (1982) 1851–1854

    Google Scholar 

  2. Crouch, C. F.; Fowler, H. W.; Spier, R. E.: The adhesion of animal cells to surfaces — The measurement of critical surface shear stress permitting attachment or causing detachment. J. Chem. Tech. Biotechnol. 35B (1985) 273–281

    Google Scholar 

  3. Nomura, H.: Ishikawa, C.: Komatsuda, T.; Ando, J.; Kamiya, A.: A disk-type apparatus for applying fluid shear stress on cultured endothelial cell. Biorheology 25 (1988) 461–470

    Google Scholar 

  4. Stathopoulos, N. A.; Hellums, J. D.: Shear stress effects on human embryonic kidney cells in vitro. Biotechnol. Bioeng. 27 (1985) 1021–1026

    Google Scholar 

  5. Born, C.: Zhang, Z.; Rubeai, M. A.; Thomas, C. R.: Estimation of disruption of animal cells by laminar shear stress. Biotechnol. Bioeng. 40 (1992) 1004–1010

    Google Scholar 

  6. Shiragami, N.: A simple and rapid method for detecting contaminants in a bioreactor. Bioprocess Eng. 5 (1990) 115–117

    Google Scholar 

  7. Cox, R. G.; Zia, I. Y. Z.; Mason, S. G.: Particle motions in sheared suspensions — XXV. Streamlines around cylinders and spheres. J. Colloid Sci. 27 (1968) 7–18

    Google Scholar 

  8. Cherry, R. S.; Kwon, K. Y.: Transient shear stresses on a suspension cell in turbulence. Biotechnol. Bioeng. 36 (1990) 563–571

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Houston, USA

    N. Shiragami

  2. Department of Bioengineering Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, 4259, Yokohama, Japan

    N. Shiragami

Authors
  1. N. Shiragami
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shiragami, N. Development of a new method for testing strength of cells against fluid shear stress. Bioprocess Engineering 10, 47–51 (1994). https://doi.org/10.1007/BF00373535

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00373535

Keywords

Search

Navigation