Summary
The erythrocyte pyrimidine 5′-nucleotidase activity (P5N) was measured in workers exposed to lead, cadmium or inorganic mercury and the effect of lead on this enzyme activity was compared with that on the classical biological parameters (ALAD, ZPP, ALAU), reflecting the interference of this metal with the heme biosynthesis pathway. P5N is inhibited by lead but not by cadmium and mercury. The correlation coefficient between log P5N and lead in blood (PbB) amount to 0.79 (n = 278; PbB ranging from 4.9 to 73.5 μg/100 ml). The response of P5N to lead closely parallels that of ALAD.
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Angle CR, McIntire MS (1977) Low-level lead inhibition of erythrocyte pyrimidine nucleotidase. Environ Res 17:296–302
Berlin A, Schaller KH (1974) European standardized method for the determination of delta-aminolevulinic acid dehydratase activity in blood. Z Klin Chem Biochem 12:389–390
Buc HA, Kaplan JC (1978) Red-cell pyrimidine 5′-nucleotidase and lead poisoning. Clin Chim Acta 87:49–55
Fendler JP, Dongradi G, Buch HA (1978) Le diagnostic infraclinique du saturnisme. Ann Méd Int 129:473–480
Gad SC, Weil CS (1982) Statistics for toxicologists. In: Hayes AW (ed) Principles and methods of toxicology. Raven Press, New York, pp 273–320
Lauwerys R, Delbroek R, Vens MD (1972) Automated analysis of delta-aminolevulinic acid in urine. Clin Chim Acta 40:443–447
Lauwerys R, Buchet JP, Roels H, Materne D (1974) Relationship between urinary delta-aminolevulinic acid excretion and the inhibition of red cell delta-aminolevulinic dehydratase by lead. Clin Toxicol 7:383–388
Lauwerys R, Buchet JP, Roels H, Hubermont G (1978) Placental transfer of lead, mercury, cadmium and carbon monoxide in women. Environ Res 15:278–289
Mohammed-Brahim B, Buchet JP, Bernard A, Lauwerys R (1984) In vitro effect of lead, mercury and cadmium on the enzymic activity of red-blood cell pyrimidine 5′-nucleotidase. Toxicol Lett 20:195–199
Paglia DE, Valentine WN, Dahlgreen JG (1975) Effects of low-level lead exposure on pyrimidine 5′-nucleotidase and other erythrocyte enzymes. Possible role of P5N in the pathogenesis of lead induced anemia. J Clin Invest 56:1164–1169
Paglia DE, Valentine WN, Fink K (1977) Lead poisoning. Further observations on erythrocyte pyrimidine 5′-nucleotidase deficiency and intracellular accumulation of pyrimidine nucleotide. J Clin Invest 60:1362–1366
Roels H, Lauwerys R, Buchet JP, Vrelust MTh (1975) Response of free erythrocyte porphyrines and urinary delta-aminolevulinic acid in men and women moderately exposed to lead. Int Arch Arbeitsmed 34:97–108
Roels H, Balis-Jacques MN, Buchet JP, Lauwerys R (1979) The influence of sex and chelation therapy on erythrocyte protoporphyria and urinary delta-aminolevulinic acid in leadexposed workers. J Occup Med 21:527–553
Sakai T, Tanagihara S, Ushio K (1980) Restauration of lead inhibited 5-aminolevulinate dehydratase activity in whole blood by heat zinc ion, and/or dithiothreitol. Clin Chem 26:625–628
Tomokuni K, Ogata M (1981) Relationship between lead concentration in blood and the activities of erythrocyte pyrimidine 5′-nucleotidase and delta-aminolevulinic dehydratase in the mice exposed to lead. Arch Toxicol 47:67–69
Valentine WN, Fink K, Paglia DE, Harris SR (1974) Hereditary hemolytic anemia with human erythrocyte pyrimidine 5′-nucleotidase deficiency. J Clin Invest 54:866–879
Valentine WN, Paglia DE, Fink K, Madokoro G (1976) Lead poisoning association with hemolytic anemia, basophilic stippling erythrocyte pyrimidine 5′-nucleotidase deficiency and intraerythrocyte accumulation of pyrimidine. J Clin Invest 58:926–932
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Mohammed-Brahim, B., Buchet, J.P. & Lauwerys, R. Erythrocyte pyrimidine 5′-nucleotidase activity in workers exposed to lead, mercury or cadmium. Int Arch Occup Environ Health 55, 247–252 (1985). https://doi.org/10.1007/BF00383757
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DOI: https://doi.org/10.1007/BF00383757