Abstract
The major cytokinins in stems of decapitated, disbudded bean plants have been identified by enzymic degradation, Sephadex LH20 and reversed phase high performance liquid chromatography, and by combined gas chromatography-mass spectrometry as 6-(4-hydroxy-3-methylbut-trans-2-enylamino)-9-β-D-ribofuranosylpurine (zeatin riboside), 6-(4-hydroxy-3-methylbutylamino)-9-β-D-ribofuranosylpurine (dihydrozeatin riboside), and the 5′-phosphates of these compounds (zeatin ribotide and dihydrozeatin ribotide). Minor cytokinins in this tissue were tentatively identified as dihydrozeatin-O-β-D-glucoside and zeatin ribotide-O-β-D-glucoside. [8-14C-]Dihydrozeatin appeared to be rapidly metabolized to dihydrozeatin ribotide when supplied to segments of stems from decapitated plants. These results are discussed in relation to the metabolism and distribution of cytokinins in the whole plant.
Similar content being viewed by others
Abbreviations
- TEAB:
-
triethyl ammonium bicarbonate
- UV:
-
ultra-violet
- GCMS:
-
gas chromatography-mass spectrometry
- HPLC:
-
high performance liquid chromatography
- TMS:
-
trimethyl silyl
References
Bieleski, R.L. (1964) The problem of halting enzyme action when extracting plant tissues. Anal. Biochem. 9, 431–442
Engelbrecht, L. (1972) Cytokinins in leaf cuttings of Phaseolus vulgaris during their development. Biochem. Physiol. Pflanz. 163, 335–343
Horgan, R., Kramers, M.R. (1979) High performance liquid chromatography of cytokinins. J. Chromatogr. 173, 263–270
Koda, Y., Okazawa, Y. (1980) Cytokinin production by Asparagus shoot apex cultured in vitro. Physiol. Plant. 49, 193–197
Letham, D.S. (1978) Cytokinins. In: Phytohormones and related compounds: a comprehensive treatise, vol. I, pp. 205–263. Letham, D.S., Goodwin, P.B., Higgins, T.J.V. (eds) Elsevier, Amsterdam
Manos, P.J., Goldthwaite, J. (1976) An improved cytokinin bioassay using cultured soybean hypocotyl sections. Plant Physiol. 57, 894–897
Miller, C.O. (1968) Naturally occurring cytokinins. In: Biochemistry and physiology of plant growth substances, pp. 33–45, Wightman, F., Setterfield, G., eds. Runge Press, Ottawa
Palmer, M.V. (1980) Cytokinin relations in the whole plant. Ph.D. thesis, University of Wales, Aberystwyth
Palmer, M.V., Scott, I.M., Horgan, R. (1981) Cytokinin metabolism in Phaseolus vulgaris L. II. Comparative metabolism of zeatin, dihydrozeatin and their O-glucosides in detached leaves. Plant Sci. Lett. (in press)
Peterson, C.S., Fletcher, R.A. (1975) Lateral bud outgrowth on excised stem segments: effect of stem. Can. J. Bot. 53, 243–248
Skene, K.G.M. (1975) Cytokinin production by roots as a factor in the control of plant growth. In: The development and function of roots. pp. 365–395. Torrey, J.G., Clarkson, D., eds. Academic Press, New York
Wang, T.L., Thompson, A.G., Horgan, R. (1977) A new cytokinin glucoside from the leaves of Phaseolus vulgaris L. Planta 135, 285–288
Wang, T.L., Horgan, R. (1978) Dihydrozeatin riboside, a minor cytokinin from the leaves of Phaseolus vulgaris L. Planta 140, 151–153
Wang, T.L., Wareing, P.F. (1979) Cytokinins and apical dominance in Solanum andigena: lateral shoot outgrowth and endogenous cytokinin levels in the absence of roots. New Phytol. 82, 19–28
Wareing, P.F., Horgan, R., Henson, I.E., Davis, W. (1977) Cytokinin relations in the whole plant. In: Plant growth regulation, pp. 147–153, Pilet, P.E., ed. Springer, Berlin Heidelberg New York
Whitty, C.D., Hall, R.H. (1974) A cytokinin oxidase in Zea mays. Can. J. Biochem. 52, 789–799
Woolley, D.J., Wareing, P.F. (1972) Role of roots, cytokinins and apical dominance in the control of lateral shoot form in Solanum andigena. Planta 105, 33–42
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Palmer, M.V., Horgan, R. & Wareing, P.F. Cytokinin metabolism in Phaseolus vulgaris L.. Planta 153, 297–302 (1981). https://doi.org/10.1007/BF00384245
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00384245