Skip to main content
SpringerLink
Log in

Basipetally polarised transport of [3H]gibberellin A1 and [14C]gibberellin A3, and acropetal polarity of [14C]indole-3-acetic acid transport, in stelar tissues of Phaseolus coccineus roots

  • Published:
Planta Aims and scope Submit manuscript

Summary

Movement of both [3H]GA1 and [14C]GA3 through root segments from P. coccineus seedlings was basipetally polarised. The basipetal/acropetal ratio of radioactivity from [3H]GA1 in agar receiver blocks was 9.2 for apical, elongating segments, and 4.0 for more basal, non-elongating segments. Polarity of gibberellin transport was restricted to the stele, and absent from cortical tissues. Transport of [14C]IAA through root segments to agar receivers was preferentially acropetal, particularly so in the stele. Despite the existence of basipetal polarity of gibberellin transport in the root, [3H]GA1 injected into cotyledons moved into and acropetally along the seedling root.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bowen, M. R., Wilkins, M. B., Cane, A. R., McCorquodale, I.: Auxin transport in roots. VIII. The distribution of radioacitvity in the tissues of zea root segments. Planta (Berl.) 105, 273–292 (1972)

    Google Scholar 

  • Bridges, I. G., Hillman, J. R., Wilkins, M. B.: Identification and localisation of auxin in primary roots of Zea mays by mass spectrometry. Planta (Berl.) 115, 189–192 (1973)

    Google Scholar 

  • Cane, A. R., Wilkins, M. B.: Auxin transport in roots. VI. Movement through different zones of Zea roots. J. exp. Bot. 21, 212–218 (1970)

    Google Scholar 

  • Crozier, A., Reid, D. M.: Do roots synthesize gibberellins? Canad. J. Bot. 49, 967–975 (1971)

    Google Scholar 

  • Davies, P. J., Mitchell, E. K.: Transport of indoleacetic acid in intact roots of Phaseolus coccineus. Planta (Berl.) 105, 139–154 (1972)

    Google Scholar 

  • Greenblatt, G. A., Jacobs, W. P.: Polar transport of gibberellic acid (GA3) through isolated internode sections of Coleus. Plant Physiol. (Lancaster) 41, Suppl., 33 (1966)

    Google Scholar 

  • Greenwood, M. S., Hillman, J. R., Wilkins, M. B.: Localization and identification of auxin in roots of Zea mays. Planta (Berl.) 109, 369–374 (1973)

    Google Scholar 

  • Haissig, B. E.: Influence of IAA on adventitious root primordia of brittle willow. Planta (Berl.) 95, 27–35 (1970)

    Google Scholar 

  • Hillman, S. K., Phillips, I. D. J.: Transport and metabolism of indol-3yl-(acetic acid-2-14C) in pea roots. J. exp. Bot. 21, 959–967 (1970)

    Google Scholar 

  • Jacobs, W. P.: The movement of plant hormones: auxins, gibberellins, and cytokinins. In: Plant Growth Substances, Proc. Seventh Int. Conf. Plant Growth Substances, Canberra, Australia (D. J. Carr, ed.), p. 701–709 (1970)

  • Jacobs, W. P., Kaldewey, H.: Polar movement of gibberellic acid through young Coleus petioles. Plant Physiol. (Lancaster) 45, 539–541 (1970)

    Google Scholar 

  • Jacobs, W. P., Pruett, P. E.: The time-course of polar movement of gibberellin through Zea roots. Amer. J. Bot. 60, 896–900 (1973)

    Google Scholar 

  • Jones, R. L., Phillips, I. D. J.: Agar-diffusion technique for estimating gibberellin production by plant organs. Nature (Lond.) 204, 497–499 (1964)

    Google Scholar 

  • Jones, R. L., Phillips, I. D. J.: Organs of gibberellin synthesis in lightgrown sunflower plants. Plant Physiol. (Lancaster) 41, 1381–1386 (1966)

    Google Scholar 

  • Jones, R. L., Phillips, I. D. J.: Effect of CCC on the gibberellin content of excised sunflower organs. Planta (Berl.) 72, 53–59 (1967)

    Google Scholar 

  • Kaldewey, H., Kraus, L.: Translocation and immobilization of radiocarbon in the hypocotyl and primary root of Gossypium hirsutum L. after application of IAA-2-14C to intact light-grown seedlings. In: Hormonal regulation in plant growth and development. (H. Kaldewey and Y. Vardar, eds.), p. 137–153. Weinheim: Verlag Chemie 1972

    Google Scholar 

  • Kende, H., Sitton, D.: The physiological significance of kinetin- and gibberellin-like root hormones. Ann. N.Y. Acad. Sci. 44, 235–243 (1967)

    Google Scholar 

  • Kirk, S. C., Jacobs, W. P.: The movement of 3-indoleacetic acid-14C in roots of Lens and Phaseolus. In: Biochemistry and Physiology of Plant Growth Substances, Proc. Sixth Int. Conf. Plant Growth Substances, Runge Press, Ottawa, Canada (F. Wightman and G. Setterfield, eds.), p. 1077–1094 (1968)

  • McComb, A. J.: The stability and movement of gibberellic acid in pea seedlings. Ann. Bot., N.S. 28, 669–687 (1964)

    Google Scholar 

  • Morris, D. A., Briant, R. E., Thomson, P. G.: The transport and metabolism of 14C-labelled indoleacetic acid in intact pea seedlings. Planta (Berl.) 89, 178–197 (1969)

    Google Scholar 

  • Most, B. H., Scott, T. K.: Transport of growth regulators in sugar cane. Plant Physiol. (Lancaster) 47, Suppl. 41 (1971)

    Google Scholar 

  • Phillips, I. D. J.: Root-shoot hormone relations. I. The importance of an aerated root system in the regulation of growth hormone levels in the shoot of Helianthus annuus. Ann. Bot., N.S. 28, 17–35 (1964a)

    Google Scholar 

  • Phillips, I. D. J.: Root-shoot hormone relations. II. Changes in endogenous auxin concentration produced by flooding of the root system in Helianthus annuus. Ann. Bot., N.S. 28, 37–45 (1964b)

    Google Scholar 

  • Phillips, I. D. J., Hartung, W.: Basipetal and acropetal transport of [3,4-3H] gibberellin A1 in short and long segments of Phaseolus coccineus second internode. Planta (Berl.) 116, 109–121 (1974)

    Google Scholar 

  • Phillips, I. D. J., Jones, R. L.: Gibberellin-like activity in bleedingsap of root systems of Helianthus annuus detected by a new dwarf pea epicotyl assay and other methods. Planta (Berl.) 63, 269–278 (1964)

    Google Scholar 

  • Pilet, P. E.: Auxin transport in roots. Lens culinaris. Nature (Lond.) 204, 561–562 (1964)

    Google Scholar 

  • Scott, T. K., Wilkins, M. B.: Auxin transport in roots. II. Polar flux of IAA in Zea roots. Planta (Berl.) 83, 323–334 (1968)

    Google Scholar 

  • Sitton, D., Richmond, A., Vaadia, Y.: On the synthesis of gibberellins in roots. Phytochemistry 6, 1101–1105 (1967)

    Google Scholar 

  • Skene, K. G. M.: Gibberellin-like substances in root exudate of Vitis vinifera. Planta (Berl.) 74, 250–262 (1967)

    Google Scholar 

  • Wilkins, M. B., Nash, L. J.: Movement of radioactivity from [3H]GA3 in geotropically stimulated coleoptiles of Zea mays. Planta (Berl.) 115, 245–251 (1974)

    Google Scholar 

  • Wilkins, M. B., Scott, T. K.: Auxin transport in roots. Nature (Lond.) 219, 1388–1389 (1968)

    Google Scholar 

  • Zweig, G. S., Yamaguchi, S., Mason, G. W.: Translocation of C14-gibberellin in red kidney bean, normal corn and dwarf corn. Advanc. Chem. Ser. 28, 112–134 (1961)

    Google Scholar 

Download references

Author information

Author notes

  1. W. Hartung

    Present address: Botanisches Institut I, Botanische Anstalten der Universität Würzburg, Mittlerer Dallenbergweg 64, D-8700, Würzburg, Federal Republic of Germany

Authors and Affiliations

  1. Department of Biological Sciences, Washington-Singer Laboratories, Exeter University, EX4 4QG, Exeter, UK

    W. Hartung & I. D. J. Phillips

Authors
  1. W. Hartung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. D. J. Phillips
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hartung, W., Phillips, I.D.J. Basipetally polarised transport of [3H]gibberellin A1 and [14C]gibberellin A3, and acropetal polarity of [14C]indole-3-acetic acid transport, in stelar tissues of Phaseolus coccineus roots. Planta 118, 311–322 (1974). https://doi.org/10.1007/BF00385581

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00385581

Keywords

Search

Navigation