Abstract
Sixteen genetic lines of peas were screened for their ability to regenerate whole plants from callus cultures. Epicotyl sections from germinating seeds were placed on callus-inducing medium; the resulting callus was subcultured monthly and was tested every other month for its regeneration ability. Six lines were found that would regenerate after 2 months' growth as callus. Four of these continued to regenerate after 4 months and, of these, two after 6 months. The cultivars “Frosty” and “Alaska” were among the lines that would not regenerate at all.
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Michigan Agricultural Experiment Station Journal Article No. 8932
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Malmberg, R.L. Regeneration of whole plants from callus culture of diverse genetic lines of Pisum sativum L.. Planta 146, 243–244 (1979). https://doi.org/10.1007/BF00388239
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DOI: https://doi.org/10.1007/BF00388239