Abstract
In an early-flowering line of pea (G2) apical senescence occurs only in long days (LD), while growth in short days (SD) is indeterminate. In SD, G2 plants are known to produce a graft-transmissible substance which delays apical senescence in related lines that are photoperiod-insensitive with regard to apical senescence. Gibberellic acid (GA3) applied to the apical bud of G2 plants in LD delayed apical senescence indefinitely, while N6-benzyladenine and α-naphthaleneacetic acid were ineffective. Of the gibberellins native to pea, GA9 had no effect whereas GA20 had a moderate senescence-delaying effect. [3H]GA9 metabolism in intact leaves of G2 plants was inhibited by LD and was restored by placing the plants back in SD. Leaves of photoperiod-insensitive lines (I-types) metabolized GA9 readily regardless of photoperiod, but the metabolites differed qualitatively from those in G2 leaves. A polar GA9 metabolite, GAE, was found only in G2 plants in SD. The level of GA-like substances in methanol extracts from G2 plants dropped about 10-fold after the plants were moved from SD to LD; it was restored by transferring the plants back to SD. A polar zone of these GA-like materials co-chromatographed with GAE. It is suggested that a polar gibberellin is synthesized by G2 plants in SD; this gibberellin promotes shoot growth and meristematic activity in the shoot apex, preventing senescence.
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Abbreviations
- GA:
-
gibberellin
- GA3 :
-
gibberellic acid
- SD:
-
short days
- LD:
-
long days
References
Bown, A.W., Reeve, D.R., Crozier, A.: The effect of light on the gibberellin metabolism and growth of Phaseolus coccineus seedlings. Planta 126, 83–91 (1975)
Cooke, R.J., Kendrick, R.E.: Phytochrome controlled gibberellin metabolism in etioplast envelopes. Planta 131, 303–307 (1976)
Davies, P.J., Proebsting, W.M., Gianfagna, T.J.: Hormonal relationships in whole plant senescence. In: Plant growth regulation, pp. 273–280, Pilet, P.E. ed. Berlin-Heidelberg-New York: Springer 1977
Durley, R.C., Crozier, A., Pharis, R.P., McLaughlin, G.E.: Chromatography of 33 gibberellins on a gradient eluted silica gel partition column. Phytochem. 11, 3029–3033 (1972)
Durley, R.C., Pharis, R.P., Zeevaart, J.A.D.: Metabolism of [3H]gibberellin A20 by plants of Bryophyllum daigremontianum under long- and short-day conditions. Planta 126, 139–149 (1975)
Ecklund, P.R., Moore, T.C.: Quantitative changes in gibberellin and RNA correlated with senescence of the shoot apex in the “Alaska” pea. Amer. J. Bot. 55, 494–503 (1968)
Evans, A., Smith, H.: Localization of phytochrome in eitoplasts and its regulation in vitro of gibberellin levels. Proc. Natl. Acad. Sci. USA 73, 138–142 (1976)
Frankland, B., Wareing, P.F.: Effect of gibberellic acid on hypocotyl growth of lettuce seedlings. Nature 185, 255–256 (1960)
Frydman, V.M., Gaskin, P., MacMillan, J.: Qualitative and quantitative analyses of gibberellins throughout seed maturation in Pisum sativum cv. Progress No. 9. Planta 118, 123–132 (1974)
Frydman, V.M., MacMillan, J.: The metabolism of gibberellins A9, A20 and A29 in immature seeds of Pisum sativum cv. Progress No. 9. Planta 125, 181–195 (1975)
Jones, R.L.: Gibberellins: their physiological role. Ann. Rev. Plant Physiol. 24, 571–598 (1973)
Lance, B., Durley, R.C., Reid, D.M., Thorpe, T.A., Pharis, R.P.: Metabolism of [3H]gibberellin A20 in light and dark grown tobacco callus cultures. Plant Physiol. 58, 387–392 (1976)
Lockhart, J.A., Gottschall, V.: Fruit-induced and apical senescence in Pisum sativum L. Plant Physiol. 36, 389–398 (1961)
MacMillan, J.: Some aspects of gibberellin metabolism in higher plants. In: Plant growth regulation, pp. 129–138, Pilet, P.E., ed. Berlin-Heidelberg-New York: Springer 1977
Marx, G.A.: Influence of genotype and environment on senescence in peas, Pisum sativum L. BioSci. 18, 505–506 (1968)
Murfet, I.C.: Flowering in Pisum: Reciprocal grafts between known genotypes. Aust. J. Biol. Sci. 24, 1089–1101 (1971)
Murfet, I.C.: Flowering in Pisum: Hr, a gene for high response to photoperiod. Heredity 31, 157–164 (1973)
Murfet, I.C., Marx, G.A.: Flowering in Pisum: Comparison of the Geneva and Hobart systems of phenotypic classification. Pisum Newslett. 8, 46–47 (1976)
Murfet, I.C., Reid, J.B.: Flowering in Pisum: evidence that gene Sn controls a graft-transmissible inhibitor. Aust. J. Biol. Sci. 26, 675–677 (1973)
Murfet, I.C., Reid, J.B.: Flowering in Pisum: the influence of photoperiod and vernalizing temperatures on the expression of genes Lf and Sn. Z. Pflanzenphysiol. 71, 323–331 (1974)
Powell, L.E., Tautvydas, K.J.: Chromatography of gibberellins on silica gel partition columns. Nature 213, 292–293 (1967)
Proebsting, W.M., Davies, P.J., Marx, G.A.: Photoperiodic control of apical senescence in a genetic line of peas. Plant Physiol. 58, 800–802 (1976)
Proebsting, W.M., Davies, P.J., Marx, G.A.: Evidence for a graft-transmissible substance which delays apical senescence in Pisum sativum L. Planta 135, 93–94 (1977)
Railton, I.D.: Studies on gibberellins in shoots of light-grown peas. II. The metabolism of tritiated gibberellin A9 and gibberellin A20 by light- and dark-grown shoots of dwarf Pisum sativum var. Meteor. Plant Sci. Lett. 3, 207–212 (1974)
Railton, I.D.: 16,17 Dihydro 16,17 dihydroxy gibberellin A9: a metabolite of [3H]-gibberellin A9 in chloroplast sonicates from Pisum sativum var. Alaska. Z. Pflanzenphysiol. 81, 323–329 (1977a)
Railton, I.D.: Gibberellin metabolism in chloroplasts of Pisum sativum L. var. Alaska. S. Afr. J. Science 73, 22–23 (1977b)
Railton, I.D., Durley, R.C., Pharis, P.: Metabolism of tritiated gibberellin A9 by shoots of dark-grown dwarf pea, cv. Meteor. Plant Physiol. 54, 6–12 (1974a)
Railton, I.D., Murofushi, N., Durley, R.C., Pharis, R.P.: Interconversion of gibberellin A20 to gibberellin A29 by etiolated seedlings and germinating seeds of dwarf Pisum sativum. Phytochem. 13, 793–796 (1974b)
Railton, I.D., Reid, D.M.: Studies on gibberellins of light-grown peas. III. Interconversion of [3H]GA9 and [3H]GA20 to other gibberellins by an in vitro system derived from chloroplasts of Pisum sativum. Plant Sci. Lett. 3, 303–308 (1974)
Reeve, D.R., Crozier, A.: An assessment of gibberellin structure-activity relationships. J. Exp. Bot. 25, 431–445 (1974)
Sponsel, V.M., Hoad, G.V., Beeley, L.J.: The biological activities of some new gibberellins (GAs) in six plant bioassays. Planta 135, 143–147 (1977)
Sponsel, V.M., MacMillan, J.: Further studies on the metabolism of gibberellins (gAs) A9, A20 and A29 in immature seeds of Pisum sativum cv. Progress No. 9. Planta 135, 129–136 (1977)
Yamane, H., Murofushi, N., Osada, H., Takanashi, N.: Metabolism of gibberellin in early immature bean seeds. Phytochem. 16, 831–835 (1977)
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Proebsting, W.M., Davies, P.J. & Marx, G.A. Photoperiod-induced changes in gibberellin metabolism in relation to apical growth and senescence in genetic lines of peas (Pisum sativum L.). Planta 141, 231–238 (1978). https://doi.org/10.1007/BF00388337
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DOI: https://doi.org/10.1007/BF00388337