Abstract
Phenylalanine ammonia-lyase from an “over-producer” strain of Coleus blumei Benth. cell cultures accumulating high levels of rosmarinic acid (RA) has been shown to possess no special feed-back sensitivity to RA or its precursors. No tyrosine-3-hydroxylase activity could be detected in culture extracts and no specific inhibitors of tyrosine incorporation into RA were found. L-α-aminooxy-β-phenyl propionic acid, however, was effective in specifically blocking phenylalanine incorporation. This block also led to an accumulation of label from tyrosine in 4-hydroxyphenyllactic acid rather than in 3,4-dihydroxyphenylalanine (DOPA) or 3,4-dihydroxyphenyllactic acid. These observations require a re-evaluation of the possible role of DOPA as a major biogenic precursor to RA.
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Abbreviations
- AOPP:
-
α-aminooxy-β-phenylpropionic acid
- DOPA:
-
3,4-dihydroxyphenylalanine
- RA:
-
rosmarinic acid (α-O-caffeoyl-3,4-dihydroxyphenyllactic acid)
- PAL:
-
L-phenylalanine ammonia-lyase (EC 4.3.1.5)
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Ellis, B.E., Remmen, S. & Goeree, G. Interactions between parallel pathways during biosynthesis of rosmarinic acid in cell suspension cultures of Coleus blumei . Planta 147, 163–167 (1979). https://doi.org/10.1007/BF00389519
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DOI: https://doi.org/10.1007/BF00389519