Summary
Small explants composed of 3–6 layers of epidermal and subepidermal cells excised from floral branches of Nicotiana tabacum are capable of de novo organogenesis. Continuous mitoses without organogenesis, or mitoses followed by the formation of floral buds, vegetative buds or roots can be obtained by varying the auxin-cytokinin-sucrose ratio in the medium. The physiological stage of the floral branches, and light and darkness had also an influence on the de novo organogenesis. Best floral-bud formation was obtained when explants were taken from plants in which the terminal bud was in the green-fruit stage, and were cultured on a medium with indole-3-acetic acid and kinetin both at 10−6 M and with 3% sucrose, in the light. For differentiation of vegetative buds, the optimal auxin/cytokinin ratio was one tenth of that for floral-bud formation, while the sucrose concentration was the same. Optimal conditions for root formation were: explants from plants in which the terminal bud carried a mature fruit; 10−7 M kinetin, 10−5 M indole-3-butyric acid, and 1% sucrose in the medium; and culture in darkness. The optimal medium for callus formation contained 5×10−6M 2,4-dichlorophenoxyacetic acid, 10−7 M kinetin and 3% sucrose.
Similar content being viewed by others
Abbreviations
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
benzyladenine
- 6γγ-DMAAP:
-
6-(γ,γ-dimethylallylamino)purine
References
Aghion-Prat, D.: Néoformation de fleurs in vitro chez Nicotiana tabacum L. Physiol. vég. 3, 229–303 (1965)
Chlyah, H.: Inter-Tissue Correlations in Organ Fragments. Organogenetic capacity of tissues excised from stem segments of Torenia fournieri Lind cultured separately in vitro. Plant Physiol., in press (1974)
Chlyah-Arnason, A., Tran Thanh Van, M.: Budding capacity of undetached Begonia rex leaves. Nature (Lond.) 218, 493 (1968)
Leroux, R.: Action de l'acide gibbérellique sur la rhizogenese de fragments de tige de Pois cultivés in vitro en présence d'auxine à l'obscurité ou à la lumière. C. R. Acad. Sci. (Paris) 266D, 106–108 (1968)
Nguyen Thi Dien, Tran Thanh Van, M.: Différenciation in vitro et de novo d'organes floraux directement à partir des ccuches minces de cellules de type épidermique de Nicotiana tabacum Wisc. 38. Etude au niveau cellulaire. Canad. J. Bót., in press (1974)
Skoog, F.: Aspects of growth interactions in morphogenesis of tobacco tissue cultures. In: Les cultures de tissues de plantes. Coll. internat. C.N.R.S. No. 193, 115–135 (1970)
Skoog, F., Miller, C. O.: Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. exp. Biol. 11, 118–131 (1957)
Tran Thanh Van, M.: In vitro control of de novo flower, bud, root, and callus differentiation from excised epidermal tissues. Nature (Lond.) 246, 44–45 (1973a)
Tran Thanh Van, M.: Direct flower formation from superficial tissue of small explants of Nicotiana labacum L. Planta (Berl.) 115, 87–92 (1973b)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tran Thanh Van, M., Dien, N.T. & Chlyah, A. Regulation of organogenesis in small explants of superficial tissue of Nicotiana tabacum L.. Planta 119, 149–159 (1974). https://doi.org/10.1007/BF00390888
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00390888