Abstract
Gluconic acid fermentation by Aspergillus niger has been investigated using untreated and treated Indian cane molasses. The yield of gluconic acid was found to be reduced using an untreated molasses medium compared to a defined medium. Hence, molasses was subjected to various pretreatment techniques. Pretreatment reduced the levels of various cations and anions. As the synthesis of gluconic acid has been observed to be influenced more by cations than anions, the effect of various metal ions, viz., copper, iron, zinc, manganese, calcium, and magnesium on the yield of gluconic acid has been critically examined in both untreated and treated cane molasses. These results have been compared with a defined medium. The yield of gluconic acid was influenced more by a combination of metal ions rather than individual ions. Potassium ferrocyanide treatment gave the most promising results compared to other treatment techniques.
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References
Milsom, P. E.; Meers, J. L.: Gluconic acid and itaconic acids, In: Moo-Young, M. (Ed.) Comprehensive Biotechnology, Vol. 3. pp 681–700. Oxford: Pergamon Press, 1985
Röhr, M.; Kubicek, C. P.; Kominek, J.: Gluconic acid, In: Rehm, H.-J.; Reed, G. (Eds.) Biotechnology, Vol. 3. pp 456–465. Weinheim: Verlag Chemie, 1983
Helmut, H.; Hans-Joachim, H.; Elizabeth, S.: Gluconic acid, In: Barabara, E.; Stephen, H.; Michael, R.; James, F. R.; Gail, S. (Eds.) Ullman's Encyclopedia of Industrial Chemistry, Vol. A12. pp 449–456. Weinheim: VCH Verlagsgesellschaft mbH, D-6940, 1989
Kundu, P. N.; Das, A.: Calcium gluconate production by a non-conventional fermentation method. Biotechnology Letters 4 (1982) 365–368
Roukas, T.; Harvey, L.: The effect of pH on production of citric and gluconic acid from beet molasses using continuous culture. Biotechnology Letters 10 (1988) 289–294
Panda, T.; Kundu, S.; Majumdar, S. K.: Studies on citric acid production by Aspergillus niger using treated Indian cane molasses. Process Biochemistry 19 (5) (1984) 183–187
Leopold, H.; Valtr, Z.: Czech. Patent No. 121, 832 (1967)
Miller, G. L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31 (1959) 426–428
Lien, O. G. Jr.: Determination of gluconolactone, galactonolactone and their free acids by hydroxamate method. Analytical Chemistry 31 (1959) 1363–1366
Rao, D. S.; Panda, T.: Comparative analysis of calcium gluconate and sodium gluconate techniques for the production of gluconic acid by Aspergillus niger. Bioprocess Engineering 8 (5/6) (1993) 203–207
Kubicek, C. P.; Hampel, W.; Röhr, M.: Manganese deficiency leads to elevated amino acid pools in citric acid accumulating Aspergillus niger. Archieves of Microbiology 123 (1979) 73–79
Röhr, M.; Kubicek, C. P.: Regulatory aspects of citric acid fermentation by Aspergillus niger. Process Biochemistry 16 (4) (1981) 34–37
Habison, A.; Kubicek, C. P.; Röhr, M.: Phosphofructokinase as a regulatory enzyme in citric acid producing Aspergillus niger. FEMS Microbiology Letters 5 (1979) 39–42
Habison, A.; Kubicek, C. P.; Röhr, M.: Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger. Biochemistry Journal 209 (1983) 669–676
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Rao, D.S., Panda, T. Critical analysis of the effect of metal ions on gluconic acid production by Aspergillus niger using a treated Indian cane molasses. Bioprocess Engineering 10, 99–107 (1994). https://doi.org/10.1007/BF00393392
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DOI: https://doi.org/10.1007/BF00393392