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Acute experimental allergic encephalomyelitis in radiation bone marrow chimeras between high and low susceptible strains of mice

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Abstract

Acute experimental allergic encephalomyelitis (EAE) is an autoimmune disease involving the central nervous system (CNS) that can be elicited in susceptible strains of mice by the subcutaneous inoculation of mouse spinal cord homogenate (MSCH) in conjunction with complete Freund's adjuvant. In order to localize the physiological compartment conveying susceptibility to mice for EAE induction, hematopoietic radiation chimeras were prepared between the highly responsive SJL and low responder B10.S strains. Upon challenge with SJL MSCH preparations, high incidence of clinical disease was exhibited by B10.S → SJL chimeras but not by SJL → B10.S mice, suggesting that non-bone-marrow-derived factors were influencing development of disease. The incidence of histological lesions in the CNS was high for virtually all experimental and control groups except normal B10.S and B10.S → B10.S reconstituted mice. In contrast, challenge with B10.S MSCH induced a high clinical incidence of EAE in both B10.S → SJL and SJL → B10.S chimeras, indicating a possible interstrain difference in the immunogenicity of relevant CNS antigens.

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Abbreviations

APC:

antigen-presenting cell

C′:

complement

CFA:

complete Freund's adjuvant

CNS:

central nervous system

EAE:

experimental allergic encephalomyelitis

MBP:

myelin basic protein

MHC:

major histocompatibility complex

MSCH:

mouse spinal cord homogenate

PBS:

phosphate-buffered saline

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Authors and Affiliations

  1. The Wistar Institute of Anatomy and Biology, 19104, Philadelphia, PA, USA

    Robert Korngold, Abbe Feldman & Lucy B. Rorke

  2. Department of Neurology, Jefferson Medical College, 19103, Philadelphia, PA, USA

    Fred D. Lublin

  3. Department of Experimental Pathology, The John Curtin School of Medical Research, 2601, Canberra, ACT, Australia

    Peter C. Doherty

Authors
  1. Robert Korngold
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  2. Abbe Feldman
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  3. Lucy B. Rorke
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  4. Fred D. Lublin
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  5. Peter C. Doherty
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Korngold, R., Feldman, A., Rorke, L.B. et al. Acute experimental allergic encephalomyelitis in radiation bone marrow chimeras between high and low susceptible strains of mice. Immunogenetics 24, 309–315 (1986). https://doi.org/10.1007/BF00395536

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  • DOI: https://doi.org/10.1007/BF00395536

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