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Changes in levels of transcripts in endosperms of castor beans treated with exogenous gibberellic acid

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Abstract

A complementary-DNA library to mRNA from castor-bean endosperm has been prepared. Three clones have been examined in detail. One of these is complementary to isocitrate-lyase mRNA. The other two clones code for proteins with M r , 42000 and 38000. All three clones have been used to measure levels of transcripts during seed germination. The three transcripts all increased during germination and the rate of their appearance is stimulated by exogenous GA3. The data strongly support the view that the action of GA3 in these seeds is to stimulate non-specifically the rate of transcription and, in turn, protein synthesis. Possible mechanisms for the action of the growth regulator are discussed.

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Abbreviations

cDNA:

complementary DNA

GA3 :

gibberellic acid

poly(A) +RNA:

polyadenylated RNA

Poly U:

polyuridylic acid

SDS-PAGE:

sodium dodecyl sulphate-polyacrylamide gel electrophoresis

tRNA:

transfer RNA

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  1. Cathie Martin

    Present address: John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK

    Cathie Martin, John R. Beeching & D. H. Northcote

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  1. Cathie Martin
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  2. John R. Beeching
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  3. D. H. Northcote
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Martin, C., Beeching, J.R. & Northcote, D.H. Changes in levels of transcripts in endosperms of castor beans treated with exogenous gibberellic acid. Planta 162, 68–76 (1984). https://doi.org/10.1007/BF00397423

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  • DOI: https://doi.org/10.1007/BF00397423

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