Abstract
A polyclonal antiserum and monoclonal antibodies have been prepared to purified pollen exines of Calocedrus decurrens Florin. The location of the antigen is in the exine, as shown by light-and electron-microscopic immunocytochemistry. The greatest reduction in antibody binding follows treatment of the exine with chemicals known to alter sporopollenin. These results provide evidence that sporopollenin is antigenic. Exines of ten species of gymnosperms and angiosperms also bound the polyclonal antiserum, indicating similarity of sporopollenin structure.
Similar content being viewed by others
References
Atkinson, A.W., Gunning, B.E.S., John, P.L.C. (1972) Sporopollenin in the cell wall of Chlorella and other algae: ultrastructure, chemistry, and incorporation of [14C]acetate studied in synchronous cultures. Planta 107, 1–32
Blakeney, A.B., Harris, P.J., Henry, R.J., Stone, B.A. (1983) A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohydr. Res. 113, 291–299
Brunner, U., Honegger, R. (1985) Chemical and ultrastructural studies on the distribution of sporopollenin-like biopolymers in six genera of lichen phycobionts. Can. J. Bot. 63, 2221–2230
Erdtman, G. (1943) An introduction to pollen analysis. Chronica Botanica, Waltham, Mass., USA
Gooday, G.W., Fawcett, P., Green, D., Shaw, G. (1973) The formation of fungal sporopollenin in the zygospore wall or Mucor mucedo: a role for the sexual carotenogenesis in the Mucorales. J. Gen. Microbiol. 74, 233–239
Hough, T., Singh, M.B., Smart, I.J., Knox, R.B. (1986) Immunofluorescent screening of monoclonal antibodies to surface antigens of animal and plant cells bound to polycarbonate membranes. J. Immunol. Methods 92, 103–108
Howlett, B.J., Clarke, A.E. (1981) Role of carbohydrate as an antigenic determinant of a glycoprotein from rye-grass (Lolium perenne) pollen. Biochem. J. 197, 707–714
König, J., Peveling, E. (1980) Vorkommen von Sporopollenin in der Zellwand des Phycobionten Trebouxia. Z. Pflanzen-physiol. 98, 459–464
May, G., Southworth, D., Dickinson, D.B. (1976) Analysis of sporopollenin isolated from pollen of Ambrosia artemisiifolia and A. trifida. Grana 15, 149–152
Prahl, A.K., Rittscher, M., Wiermann, R. (1986) New aspects of sporopollenin biosynthesis. In: Biotechnology and ecology of pollen, pp. 313–318, Mulcahy, D., Mulcahy, G., Ottaviano, E., eds. Springer, New York
Shaw, G. (1970) Sporopollenin. In: Phytochemical phylogeny, pp. 31–58, Harborne, J., ed. Academic Press, New York
Shaw, G. (1971) Chemistry of sporopollenin. In: Sporopollenin, pp. 305–348, Brooks, J., Grant, P.R., Muir, M., Van Gijzel, P., Shaw, G., eds. Academic Press, New York
Shaw, G., Yeadon, A. (1964) Chemical studies on the constitution of some pollen and spore membranes. Grana Palynol. 5, 247–252
Singh, M.B., Knox, R.B. (1985) Grass pollen allergens: antigenic relationships detected using monoclonal antibodies and dot blotting immunoassay. Int. Arch. allergy Appl. Immunol. 78, 300–304
Southworth, D. (1969) Ultraviolet absorption spectra of pollen and spore walls. Grana Palynol. 9, 5–15
Southworth, D. (1974) Cytochemical reactivity of pollen walls. J. Histochem. Cytochem. 21, 73–80
Southworth, D. (1974) Solubility of pollen exines. Am. J. Bot. 61, 36–44
Southworth, D. (1988) Isolation of exines from gymnosperm pollen. Am. J. Bot. 75, 15–21
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Southworth, D., Singh, M.B., Hough, T. et al. Antibodies to pollen exines. Planta 176, 482–487 (1988). https://doi.org/10.1007/BF00397654
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00397654