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Marker profiles of human leukemia and lymphoma cell lines

  • Symposium on Recent Data from the Characterization of Malignant Lymphoma, November 7–8, 1980 in Kiel
  • Cytogenetics, Lymphoma Cell Lines, and Monoclonal Antibodies
  • Published:
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Summary

By means of the multiple marker analysis, a total of 55 human leukemia-lymphoma cell lines which included 15 T-cell, 30 B-cell, four myelomonocytic-cell, and six non-T, non-B cell lines was characterized for their marker profiles. The multiple markers used included a number of cell surface markers as detected by either rosette or immunofluorescence tests, enzyme assays, cytogenetic analysis, and certain functional assay. Based on the criteria previously defined it was found that all the cell lines were proved to represent original leukemia-lymphoma of ALL, AML, CLL, CML in blastic phase or variety of lymphomas. The monoclonality, a “frozen” state at a specific stage of differentiation-maturation, and cytogenetic marker in each leukemia-lymphoma cell line were remarkable common properties and were stable for years of cultivation. Similar, if not identical, general characteristics were observed in the study on 344 cases of uncultured fresh leukemia-lymphomas by the multiple marker analysis. While no single marker specific to any type of tumor was found, the study offers not only a basis for better understanding of the biology of leukemia-lymphoma but also an insight into normal hematopoietic cell differentiation in man.

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Authors and Affiliations

  1. Dept. of Immunology Roswell Park Memorial Institute, New York State Department of Health, 666 Elm Street, 14263, Buffalo, NY, USA

    J. Minowada, K. Sagawa, I. Kubonishi, M. S. Lok & E. Tatsumi

  2. Dept. of Medical Oncology Roswell Park Memorial Institute, New York State Department of Health, 66 Elm Street, 14263, Buffalo, NY, USA

    H. Koshiba & T. Han

  3. Dept. of Experimental Therapeutics Roswell Park Memorial Institute, New York State Department of Health, 666 Elm Street, 14263, Buffalo, NY, USA

    B. I. S. Srivastava

  4. Dept. of Neoplastic Diseases, Mount Sinai School of Medicine, 10029, New York, NY, USA

    T. Ohnuma

Authors
  1. J. Minowada
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  2. H. Koshiba
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  3. K. Sagawa
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  4. I. Kubonishi
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  5. M. S. Lok
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  6. E. Tatsumi
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  7. T. Han
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  8. B. I. S. Srivastava
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  9. T. Ohnuma
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Additional information

Supported by USPHS grants CA-14413, CA-17609, CA-25865, AI-08899, and contract 31-CB74165 from the National Cancer Institute and National Institute of Allergy and Infectious Diseases

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Minowada, J., Koshiba, H., Sagawa, K. et al. Marker profiles of human leukemia and lymphoma cell lines. J Cancer Res Clin Oncol 101, 91–100 (1981). https://doi.org/10.1007/BF00405069

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  • DOI: https://doi.org/10.1007/BF00405069

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