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Nickel, a component of factor F 430 from Methanobacterium thermoautotrophicum

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Abstract

Methanobacterium thermoautotrophicum, growing on medium supplemented with 2 μmol 63NiCl2/l, was found to take up 1.2 μmol 63Ni per g cells (dry weight). More than 70% of the radioisotope was incorporated into a compound, which dissociated from the protein fraction after heat treatment, was soluble in 70% acetone, and could be purified by chromatography on QAE-Sephadex A-25, Sephadex G-25, and DEAE cellulose. The purified 63Ni labelled compound had an absorption spectrum and properties identical to those of factor F 430 and is therefore considered to be identical with factor F 430.

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Authors and Affiliations

  1. Fachbereich Biologie/Mikrobiologie, Philipps-Universität Marburg, D-3550, Marburg, Germany

    Gabriele Diekert, Birte Klee & Rudolf K. Thauer

Authors
  1. Gabriele Diekert
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  2. Birte Klee
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  3. Rudolf K. Thauer
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Additional information

Factor F 430, a compound of molecular weight higher than 1000 with an absorbance maximum at 430 nm, has recently been purified from Methanobacterium thermoautotrophicum (Gunsalus and Wolfe, 1978). The structure and function of this compound are not yet known.

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Diekert, G., Klee, B. & Thauer, R.K. Nickel, a component of factor F 430 from Methanobacterium thermoautotrophicum . Arch. Microbiol. 124, 103–106 (1980). https://doi.org/10.1007/BF00407036

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  • DOI: https://doi.org/10.1007/BF00407036

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