Abstract
An analysis of the compartmentation and fluxes of inorganic phosphate in isolated cladophyll cells from Asparagus officinalis was made in parallel with an ultrastructural study. The elution pattern of labelled inorganic phosphate (which indicates that the asparagus cells are behaving as a system of three compartments in series) was used to quantify the fluxes between the vacuole, cytoplasm and free space. A relaxation time of 198 min was calculated for inorganic phosphate exchange between the vacuole and cytoplasm. It is, therefore, suggested that the vacuole serves to buffer the cytoplasmic inorganic phosphate concentration in the long term. However, in the short term, exchange with the vacuole will not appreciably affect the cytoplasmic inorganic phosphate concentration and thus the partitioning of photosynthetically fixed carbon.
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Abbreviations
- Hepes:
-
4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid
- Pi :
-
inorganic phosphate
References
Bieleski, R.L. (1968) Levels of phosphate esters in Spirodela. Plant Physiol. 43, 1297–1308
Bieleski, R.L. (1969) Phosphorous compounds in translocating pholem. Plant Physiol. 44, 497–502
Bieleski, R.L. (1973) Phosphate pools, phosphate transport and phosphate availability. Annu. Rev. Plant Physiol. 24, 225–253
Bieleski, R.L., Laties, G.G. (1963) Turnover rates of phosphate esters in fresh and aged slices of potato tuber tissue. Plant Physiol. 38, 586–594
Colman, B., Mawson, B.T., Espie, G.S. (1979) The rapid isolation of photosynthetically active mesophyll cells from Asparagus cladophylls. Can. J. Bot. 57, 1505–1510
Cram, W.J. (1968) Compartmentation and exchange of chloride in carrot root tissue. Biochim. Biophys. Acta 163, 339–353
Cram, W.J. (1975) Storage tissues. In: Ion transport in plant cells and tissues. Barker, D.A., Hall, J.L., eds. North Holland Publishing Co., Amsterdam London
Cséke, C., Buchanan, B.B. (1983) An enzyme synthesising fructose-2,6-bisphosphate occurs in leaves and is regulated by metabolic effectors. FEBS Lett. 155, 139–142
Findlay, G.P., Hope, A.B., Walker, N.A. (1971) Quantisation of a flux ratio in chlorophytes. Biochim. Biophys. Acta 233 155–162
Foyer, C.H., Walker, D.A., Spencer, C., Mann, B. (1982) Observations on the phosphate status and intracellular pH of intact cells protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance. Biochem. J. 202, 429–434
Greenway, H., Klepper, B. (1968) Phosphorous transport to the xylem and its regulation by water flow. Planta 83, 119–136
Harbron, S., Foyer, C.H., Walker, D.A. (1981) The purification and properties of sucrose-phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis. Arch. Biochem. Biophys. 212, 237–246
Jullien, M. (1983) Medium-term preservation of mesophyll cells isolated from Asparagus officinalis L.: development of a simple method by storage at reduced temperature. Plant Cell Tissue Organ Culture 2, 305–316
Leech, R.M. (1977) Subcellular fractionation techniques in enzyme distribution studies. In: Regulation of enzyme synthesis and activity in higher plants, pp. 289–327, Smith, H., ed. Academic Press, London
Mackinney, G. (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–323
MacRobbie, E.A.C. (1969) Ion fluxes to the vacuole of Nitella translucens. J. Exp. Bot. 20, 236–256
Pallaghy, C.K., Scott, B.I.H. (1969) The electrochemical state of cells of broad bean roots. II. Potassium kinetics in excised root tissue. Aust. J. Biol. Sci. 22, 585–600
Pitman, M.G. (1963) The determination of the salt relations of the cytoplasmic phase in cells of beetroot tissue. Aust. J. Biol. Sci. 16, 647–668
Rebeille, F., Bligny, R., Martin, J.-B., Douce, R. (1983) Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch. Biochem. Biophys. 225, 143–148
Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212
Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43
Tolbert, N.E., Weibe, H. (1955) Phosphorous and sulphur compounds in plant xylem sap. Plant Physiol 30, 499–504
Walker, D.A. (1980) Physiological aspects of crop productivity. Proc. 15th Colloq. Int. Potash Institute, Bern, pp. 195–207
Walker, N.A., Pitman, M.G. (1976) Measurement of fluxes across membranes. In: Encyclopedia of plant physiology, N.S., vol. 2A: Transport in plants. IIA. Cells, pp. 93–126, Lüttge, U., Pitman, M.G., eds. Springer, Berlin Heidelberg New York
Weibel, E.R., Kistler, G.S., Scherle, W.F. (1966) Practical stereological methods for morphometric cytology. J. Cell Biol. 30, 23–38
Woodrow, I.E., Murphy, D.J., Walker, D.A. (1983) Regulation of photosynthetic carbon metabolism. The effect of inorganic phosphate on stromal sedoheptulose 1,7-bisphosphatase. Eur. J. Biochem. 132, 121–123
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Woodrow, I.E., Raymond Ellis, J., Jellings, A. et al. Compartmentation and fluxes of inorganic phosphate in photosynthetic cells. Planta 161, 525–530 (1984). https://doi.org/10.1007/BF00407084
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DOI: https://doi.org/10.1007/BF00407084