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The use of glutamic acid for the isolation and identification of Clostridium cochlearium and Cl. tetanomorphum

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Summary

A simple method has been developed to test the ability of anaerobes to ferment glutamic acid. This has been applied to a systematic study of seventeen strains of anaerobic spore-formers isolated from soil with the aid of enrichment cultures containing glutamic acid as the main source of carbon and energy. The twelve glutamic acid fermenting strains were found to be similar in their morphological and physiological characters except with respect to the utilization of glucose and pyruvate (with one strain also lactose, mannitol and salicin). These organisms are identified with two previously described species, Cl. cochlearium and Cl. tetanomorphum. The five glutamic acid negative strains differ distinctly from the positive strains also with respect to other characters. The ability to ferment glutamic acid therefore appears to be of taxonimic value.

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Authors and Affiliations

  1. Division of Plant nutrition, University of California, Berkeley, California

    H. Albert Barker

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  1. H. Albert Barker
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Barker, H.A. The use of glutamic acid for the isolation and identification of Clostridium cochlearium and Cl. tetanomorphum. Archiv. Mikrobiol. 10, 376–384 (1939). https://doi.org/10.1007/BF00407259

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  • DOI: https://doi.org/10.1007/BF00407259

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