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Breakdown of chitin by Cytophaga johnsonii

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Summary

Decomposition of chitin by Cytophaga johnsonii was investigated. Unlike other chitinolytic bacteria, some strains of C. johnsonii did not liberate chitinase extracellularly; instead the cells of such strains had need for close contact with the chitin particles in order to hydrolyze them. The cell-free extract of one of these strains, viz., C. johnsonii C35 did show presence of chitinase. The remaining strains liberated an extracellular chitinase and a chitobiase. The partially purified chitinase from the culture filtrates of C. johnsonii C31 was most active at pH 6.3–6.5 and at 40°C.

Metabolism of N-acetylglucosamine, a product of chitin hydrolysis, by C. johnsonii C35 and C31 was investigated. The nature of some of the products formed and the steps involved in their transformations by the resting cells and the cell-free extracts suggested that N-acetylglucosamine first gets deacetylated to glucosamine before it is further oxidized to glucosaminic acid by these strains. Both strains were also able to dehydrogenate gluconate to 2-ketogluconate, the former being in all probability the product of deamination of glucosaminic acid.

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References

  • Anderson, R. L., Ordal, E. J.: Cytophaga succinicans sp. n., a facultatively anaerobic aquatic myxobacterium. J. Bact. 81, 130–138 (1961).

    PubMed  Google Scholar 

  • Berger, L. R., Reynolds, D. M.: The chitinase system of a strain of Streptomyces griseus. Biochim. biophys. Acta (Amst.) 29, 522–534 (1968).

    Article  Google Scholar 

  • Datta, A. G., Katznelson, H.: Oxidation of 2,5-diketogluconate by a cell-free enzymes preparation from Acetobacter melanogenum. Nature (Lond.) 179, 153–154 (1957).

    Google Scholar 

  • Jeuniaux, C.: Purification of a streptomyces chitinase. Biochem. J. 66, 29 p. (1957).

    Google Scholar 

  • —: Recherches sur les chitinases. II. Purification de la chitinase d'un streptomycete, et séparation électrophorétique de principes chitinolytiques, distincts. Arch. intern. Physiol. Biochim. 67, 597–617 (1969).

    Google Scholar 

  • Kulka, D., Walker, T. K.: The ketogenic activities of Acetobacter species in a glucose medium. Arch. Biochem. 50, 169–179 (1954).

    PubMed  Google Scholar 

  • Kvamme, E., Hellman, L.: Isolation of pyruvic and α-Ketoglutaric acid from blood and tissues in the presence of carbon-14 acetate. Analyt. Chem. 26, 1995–1997 (1954).

    Google Scholar 

  • Levvy, G. A., Conchie, J.: Mammalian glycosidase and their inhibition by aldonolactones. In: Methods in Enzymology, Vol. VIII, pp. 571–584, ed. by E. F. Neufeld and V. Ginsburg. New York: Academic Press 1966.

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).

    PubMed  Google Scholar 

  • Narrod, S. A., Wood, W. A.: Carbohydrate oxidation by Pseudomonas fluorescens. V. Evidence for gluconokinase and 2-ketogluconokinase. J. biol. Chem. 220, 45–55 (1956).

    PubMed  Google Scholar 

  • Partridge, S. M.: Filter-paper partition chromatography of sugars. 1. General description and application to the qualitative analysis of sugars in apple juices, egg white and foetal blood of sheep. Biochem. J. 42, 238–249 (1948).

    Google Scholar 

  • —: Aniline hydrogen phthalate as sparying reagent for chromatography of sugars. Nature (Lond.) 164, 443 (1949).

    Google Scholar 

  • Payne, W. J., Kieber, R. J.: The chromatographic determination of glucosamine with ninhydrin. Arch. Biochem. 52, 1–4 (1954).

    PubMed  Google Scholar 

  • Peterson, E. A., Sober, H. A.: In: Methods in enzymology, vol. V, pp. 3–27, ed. by S. P. Colowick and N. O. Kaplan. New York: Academic Press 1962.

    Google Scholar 

  • Stanier, R. Y.: The cytophaga groups: A contribution to the biology of myxobacteria. Bact. Rev. 6, 143–196 (1942).

    Google Scholar 

  • —: Studies on nonfruiting myxobacteria I. Cytophaga johnsonae, N. sp., A chitin decomposing myxobacterium. J. Bact. 53, 297–315 (1947).

    Google Scholar 

  • Tracey, M. V.: Chitin. In: Modern methods of plant analysis, vol. 2, pp. 264–274, ed. by K. Paech and M. V. Tracey. Berlin-Göttingen-Heidelberg: Springer 1955.

    Google Scholar 

  • Veldkamp, H.: A study of the aerobic decomposition of chitin by micro-organisms. Mededel. Landbouwhogesch. Wageningen 55, 127–174 (1955).

    Google Scholar 

  • Wieland, Th., Fischer, E.: Papierchromatographie von α-Ketosäuren. Naturwissenschaften 36, 219 (1949).

    Google Scholar 

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Authors and Affiliations

  1. Microbiology and Pharmacology Laboratory, Indian Institute of Science, 12, Bangalore, India

    Nirmala Sundarraj & J. V. Bhat

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  1. Nirmala Sundarraj
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  2. J. V. Bhat
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Sundarraj, N., Bhat, J.V. Breakdown of chitin by Cytophaga johnsonii . Archiv. Mikrobiol. 85, 159–167 (1972). https://doi.org/10.1007/BF00409298

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  • DOI: https://doi.org/10.1007/BF00409298

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