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Lysis of growing fissin-yeast cells induced by aculeacin A, a new antifungal antibiotic

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Abstract

Cells of Schizosaccharomyces pombe grown in the presence of aculeacin A, a peptide antibiotic, were lysed resulting the death of cells. Under high osmolarity, the cellular lysis induced by aculeacin A was considerably reduced. The use of synchronous-culture systems distinguished cell elongation from cell division revealed that the sites of aculeacin A-induced lysis on the fission yeast were the end(s) and the cell plate region, corresponded to the regions of the cell wall synthesis. Aculeacin A-resistant survivors exhibited morphological alterations which were swollen at one or both ends of the cell and appeared drumstick or dumbbel like; the wall of the bulge region was observed to be stained with a fluorescent brightener, as well as that of the cell plate region. These effects of aculeacin A are discussed as compared with effects of 2-deoxy-D-glucose.

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Authors and Affiliations

  1. Gify College of Pharmacy, 5-6-1, 502, Mitahora-higashi, Gifu

    Machiko Miyata & Jiro Kitamura

  2. Department of Biology, Faculty of Science, Nagoya University, Chikusa-ku, 464, Nagoya, Japan

    Hisao Miyata

Authors
  1. Machiko Miyata
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  2. Jiro Kitamura
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  3. Hisao Miyata
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Miyata, M., Kitamura, J. & Miyata, H. Lysis of growing fissin-yeast cells induced by aculeacin A, a new antifungal antibiotic. Arch. Microbiol. 127, 11–16 (1980). https://doi.org/10.1007/BF00414349

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  • DOI: https://doi.org/10.1007/BF00414349

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