Abstract
A strain of Pseudomonas putida capable of utilizing both stereoisomers of phenylglycine as the sole carbon and energy source was isolated from soil. No phenylglycine racemase was detected in cells grown on either stereoisomer. In an initial reaction each steroisomer of phenylglycine was transaminated yielding phenylglyoxylate which was further metabolized via benzaldehyde to benzoate. Subsequently, benzoate was further degraded via an ortho-cleavage of catechol.
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Abbreviations
- HPLC:
-
high-performance liquid chromatography
References
Bhat SG, Ramanarayanan M, Vaidyanathan CS (1973) Mandelic acid-4-hydroxylase, a new inducible enzyme from Pseudomonas convexa. Biochem Biophys Res Commun 52:834–842
Boeker EA, Snell EE (1972) Amino acid decarboxylases. In: Boyer PD (ed) The enzymes, vol 6. Academic Press, London New York, pp 217–253
Fujioka M, Morino Y, Wada H (1970) Metabolism of phenylalanine (Achromobacter eurydice). In: Tabor H, White-Tabor C (eds) Methods in enzymology, vol 17. Academic Press, London New York, pp 585–589
Guroff G, Ito T (1964) Phenylalanine hydroxylation by Pseudomonas species (ATCC 11299a). J Biol Chem 240:1175–1184
Hayaishi O, Katagiri M, Rothberg S (1957) Studies on oxygenases: Pyrocatechase. J Biol Chem 229:905–920
Hegeman GD (1970) Mandelate decarboxylase (Pseudomonas putida). In: Tabor H, White-Tabor C (eds) Methods in enzymology, vol 17. Academic Press, London New York, pp 674–678
Hegeman GD, Rosenberg EY, Kenyon GL (1970) Mandelic acid racemase from Pseudomonas putida. Purification and properties of the enzyme. Biochem 9:4029–4036
Hotta SS (1968) Oxidative metabolism of isolated brain mitochondria: changes caused by aminooxyacetate. Arch Biochem Biophys 127:132–139
Hummel W, Weiss N, Kula M-R (1984) Isolation and characterization of a bacterium possessing l-phenylalanine dehydrogenase activity. Arch Microbiol 137:47–52
Kojima Y, Itada N, Hayaishi O (1961) Metapyrocatechuase: a new catechol-cleaving-enzyme. J Biol Chem 236:2223–2228
Koyama H (1982) Purification and characterization of a novel l-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501. J Biochem 92:1235–1240
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Ogata K, Uchiyama K, Yamada H (1966) Microbial formation of cinnamic acid from phenylalanine. Agr Biol Chem 30:311–312
Reiner AM, Hegeman GD (1971) Metabolism of benzoic acid by bacteria. Accumulation of (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid by a mutant strain of Alcaligenes eutrophus. Biochem 10:2530–2536
Soda K, Osumi T (1969) Crystalline amino acid racemase with low substrate specificity. Biochem Biophys Res Commun 35:363–368
Stevenson IL, Mandelstam J (1965) Induction and multisensitive end-product repression in two converging pathways degrading aromatic substances in Pseudomonas fluorescens. Biochem J 96:354–362
Tangen O, Fonnum F, Haavaldsen R (1965) Separation and purification of aromatic amino acid transaminases from rat brain. Biochim Biophys Acta 96:82–90
Thorne CB, Gomez CG, Housewright RD (1955) Transamination of d-amino acids by Bacillus subtilis. J Bacteriol 69:357–362
Vishniac W, Santer M (1957) The thiobacilli. Bacteriol Rev 21:195–213
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van den Tweel, W.J.J., Smits, J.P. & de Bont, J.A.M. Microbial metabolism of d- and l-phenylglycine by Pseudomonas putida LW-4. Arch. Microbiol. 144, 169–174 (1986). https://doi.org/10.1007/BF00414730
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DOI: https://doi.org/10.1007/BF00414730