Abstract
Bacillus subtilis AU-1 was found to produce carboxymethylcellulase (CMCase) and Avicelase activities in the culture supernatant when grown on a variety of carbohydrates as major carbon source. Maximum CMCase production was obtained in a liquid medium containing 0.2% D (+) raffinose as inducer, 0.5% each of yeast extract, casamino acids and proteose peptone at 50 °C and at an initial pH of 6.0. CMCase activity was detected at early log phase of growth, and reached the maximum level at early stationary phase of growth which occurred at the 10th hour of cultivation. The optimal temperature for CMCase activity was 65 °C, and the enzyme was highly stable up to 60 °C. CMCase synthesis was subjected to catabolite repression by glucose and cellobiose.
Similar content being viewed by others
References
Au, K. S. & Chan, K. Y. (1986) Carboxymethylcellulase production by Bacillus subtilis. Microbios 48: 93–108
Fogarty, W. M., Griffin, P. J. (1973) Some preliminary observations on the production and properties of a cellulolytic enzyme elaborated by Bacillus polymyxa. Biochem. Soc. Trans. 1: 44–45
Herr, D. (1979) Secretion of cellulase and β-glucosidase by Trichoderma viride ITCC-1433 in submerged culture on different substrates. Biotechnol. Bioeng 21: 1361–1371
Horikoshi, K., Nakao, M., Kurono, Y. & Sashihara, N. (1984) Cellulases of an alkalophilic Bacillus strain isolated from soil. Can. J. Microbiol. 30: 774–779
Knosel, D. (1971) Continued investigation for pectolytic and cellulolytic activity of different Bacillus species. Zb. 1 Bakt. Abt. II 126: 604–6099
Lee, R. H., Blackburn, T. H. (1975) Cellulase production by a thermophilic Clostridium species. Appl. Microbiol. 30: 346–353
Loewenber, J., Chapman, C. M. (1977) Sophorose metabolism and cellulase induction in Trichoderma. Arch. Microbiol. 113: 61–64
Mandels, M. (1982) Cellulases. In: Tsau, G. T. (ed.) Annual reports on fermentation processes, Vol 5, Academic Press, Inc. New York p. 35–78
Ng, T. K., Zeikus, J. G. (1981) Comparison of extracellular cellulase activities of Clostridium thermocellum LQR1 and Trichoderma reesei QM9414. Appl. Environ. Microbiol. 42: 231–240
Priest, F. G. (1977) Extracellular enzyme synthesis in the genus Bacillus. Bacteriol. Rev. 41: 711–753
Reese, E. T., Levinson, H. S. (1952) A comparative study of the breakdown of cellulose by microorganisms. Physiol. Plant 5: 345–367
Reese, E. T., Lola, J. E., Parrish, F. W. (1969) Modified substrate and modified products as inducers of carbohydrases. J. Bacteriol. 100: 1151–1154
Reynolds, P. H., Sissons, C. H., Daniel, R. M., Morgan, H. W. (1986) Comparison of cellulolytic activities in Clostridium thermocellum and three thermophilic, cellulolytic anaerobes. Appl. Environ. Microbiol. 51: 12–17
Robson, L. M., Chambliss, G. H. (1984) Characterization of the cellulolytic activity of a Bacillus isolate. Appl. Environ. Microbiol. 47: 1039–1046
Rowe, J. J., Goldverg, D., Amelunsen, R. E. (1975) Development of defined and minimal media for the growth of Bacillus stearothermophilus. J. Bacteriol. 124: 279–285
Saddler, J. N., Khan, A. W. (1980) Cellulase production by Acetivibrio cellulolyticus. Can. J. Microbiol. 26: 760–765
Saddler, J. N., Khan, A. W., Martin, S. M. (1980) Regulation of cellulase synthesis in Acetivibrio cellulolyticus. Microbios 28: 97–106
Sippola, M., Mantsala, P. (1981) Co-production of several exo-enzymes in Bacillus subtilis (α-amylase, sucrase, neutral protease, alkaline protease, cellulase). FEMS Microbiol. Lett. 10: 303–316
Stewart, B. J., Leatherwood, J. M. (1976) Derepressed synthesis of cellulase by Cellulomonas. J. Bacteriol. 128: 609–615
Thayer, D. W. (1978) Carboxymethylcellulase produced by facultative bacteria from the hind-gut of the termite Reticulitermes hesperus. J. Gen. Microbiol. 106: 13–18
Whittle, D. J., Kiburn, D. G., Wren, R. A. J., Miller, R. C. (1982) Molecular cloning of Cellulomonas fimi cellulase gene on Escherichia coli. Gene 17: 139–145
Yamane, K., Suzuki, H., Yamaguchi, K., Tsukada, M., Nisizawa, K. (1965) Formation of cellulase in Pseudomonas fluorescens var. cellulosa. J. Ferment. Technol. 10: 721–730
Zemek, J., Augustin, J., Borriss, R., Kuniak, D., Svabova, M., Pacova, Z. (1981) Polysaccharide-hydrolyzing enzymes in the genus Bacillus. Folia Microbiol 26: 403–407.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chan, KY., Au, K.S. Studies on cellulase production by a Bacillus subtilis . Antonie van Leeuwenhoek 53, 125–136 (1987). https://doi.org/10.1007/BF00419509
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00419509