Summary
A novel type of regulatory mutation for galactose metabolism in Saccharomyces cerevisiae is described. The mutation named gal11 was recessive, non-allelic to GAL4, GAL80, GAL2, or GAL3, and unlinked to the gene cluster of GAL1, GAL10, and GAL7. It caused a ‘coordinate’ reduction of galactokinase, galactose-1-P uridylyl transferase, and UDP-glucose 4-epimerase by a factor of more than 5, rendering the mutant cells galactose-nonfermenting. The effect of the mutation was manifested not only in cells grown on galactose but also in cells constitutively synthesizing the galactose-metabolizing enzymes.
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References
Adams BG (1972) J Bacteriol 111:308–315
Bassel J, Mortimer R (1971) J Bacteriol 108:179–183
Buttin G (1963) J Mol Biol 7:164–182
Douglas HC (1961) Biochim Biphys Acta 52:209–211
Douglas HC, Condie F (1954) J Bacteriol 68:662–670
Douglas HC, Hawthorne DC (1964) Genetics 49:837–844
Douglas HC, Hawthorne DC (1966) Genetics 54:911–916
Douglas HC, Pelroy G (1963) Biochim Biophys Acta 68:155–156
Fukasawa T, Obonai K, Segawa T, Nogi Y (1980) J Biol Chem 255:2705–2707
Gornall AG, Bardawill CJ, David MM (1949) J Biol Chem 177: 751–766
Hopper JE, Broach JR, Rowe LB (1978) Proc Nat Acad Sci USA 75:2878–2882
Kew OM, Douglas HC (1976) J Bacteriol 125:33–41
Matsumoto K, Toh-e A, Oshima Y (1978) J Bacteriol 134:446–457
Nogi Y, Matsumoto K, Toh-e A, Oshima Y (1977) Mol Gen Genet 152:137–144
Perlman D, Hopper JE (1979) Cell 16:89–95
Sherman F, Fink GR, Lawrence CW (1974) Laboratory manual for a course of Methods in yeast genetics. Cold Spring Harbor Laboratory for Quantitative Biology, Cold Spring Harbor, New York
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This work was supported in part by a grant (Project No. 410712) from the Ministry of Education, Culture, and Science or Japan
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Nogi, Y., Fukasawa, T. A novel mutation that affects utilization of galactose in Saccharomyces cerevisiae . Curr Genet 2, 115–120 (1980). https://doi.org/10.1007/BF00420623
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DOI: https://doi.org/10.1007/BF00420623