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Hypoxanthine: Guanine phosphoribosyltransferase mutants in Saccharomyces cerevisiae

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Summary

Yeast mutants lacking activity of the enzyme hypoxanthine: guanine phosphoribosyltransferase (H:GPRT) have been isolated by selecting for resistance to 8-azaguanine in a strain carrying the wild type allele, ade4 + of the gene coding for amidophosphoribosyltransferase (PRPPAT), the first enzyme of de novo purine synthesis. The mutants excrete purines and are cross-resistant to 8-azaadenine. They are recessive and represent a single complementation group, designated hpt1. Ade4-su, a prototrophic allele of ade4 with reduced activity of PRPPAT, is epistatic to hpt1, suppressing purine excretion and resistance to azaadenine but not resistance to azaguanine. The genotype ade2 hpt1 does not respond to hypoxanthine. Hpt1 complements and is not closely linked to the purine excreting mutants pur1 to pur5. Hpt1 and pur6, a regulatory mutant of PRPPAT, are also unlinked but do not complement, suggesting a protein-protein interaction between H:G-PRT and PRPPAT. Mycophenolic acid (MPA), an inhibitor of de novo guanine nucleotide synthesis, inhibits the growth of hpt1 and hpt1 +. Xanthine allows both genotypes to grow in the presence of MPA whereas guanine only allows growth of hpt1 +. Activity of A-PRT, X-PRT and H:G-PRT is present in hpt +. Hpt1 lacks activity of H:G-PRT but has normal A-PRT and X-PRT.

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Authors and Affiliations

  1. Department of Biology, University of Winnipeg, R3B 2E9, Winnipeg, Manitoba, Canada

    Robin A. Woods & Darlene G. Roberts

  2. Department of Pediatrics, School of Medicine, University of California, San Diego, 92093, La Jolla, CA, USA

    Theodore Friedman, Douglas Jolly & David Filpula

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  1. Robin A. Woods
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  2. Darlene G. Roberts
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  3. Theodore Friedman
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  4. Douglas Jolly
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  5. David Filpula
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Communicated by G. Fink

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Woods, R.A., Roberts, D.G., Friedman, T. et al. Hypoxanthine: Guanine phosphoribosyltransferase mutants in Saccharomyces cerevisiae . Molec. Gen. Genet. 191, 407–412 (1983). https://doi.org/10.1007/BF00425755

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  • DOI: https://doi.org/10.1007/BF00425755

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