Abstract
Three isolates of Gliocladium virens (G1, G2 and G3) and two of Trichoderma longibrachiatum (T1 and T2) were screened against isolates of three soilborne plant pathogens namely Rhizoctonia solani, Sclerotium rolfsii and Pythium aphanidermatum. G. virens exhibited stronger hyperparasitism and wider biological spectrum than T. longibrachiatum. Further, similarities as well as variation was observed in the ability of the various isolates to invade the test pathogens in dual culture. For the hyperparasites, acidic pH range (5.0 to 5.5) favoured both growth and spore germination. The hyperparasites made direct contact with the pathogens followed by varied modes of attack invariably leading to cell disruption. Antagonists, G1 and G3 revealed strong antibiosis while T2 showed moderate effect. All the isolates produced enhanced levels of lytic enzymes adaptively and there were marked differences among them. However, no correlation was observed between these attributes and the hyperparasitic potential of the various isolates in dual culture. The relevance and the role of enzymes and toxic metabolite(s) in the antagonism of G. virens and T. longibrachiatum to these pathogens are discussed.
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References
Aluko MO, Hering TF. The mechanisms associated with the antagonistic relationship between Corticium solani and Gliocladium virens. Trans Br mycol Soc 1970; 55: 173–99.
Baker KF. Evolving concepts of biological control of plant pathogens. Ann Rev Phytopathol 1987; 25: 67–85.
Baker R. Mycoparasitism: ecology and physiology. Can J Plant Pathol 1987; 9: 370–9.
Bell DK, Wells HD, Markham CR. In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopath 1982; 72: 379–82.
Brain PW, Hemming HG. Gliotoxin, a fungistatic metabolic product of Trichoderma viride. Ann Appl Biol 1945; 32: 214–20.
Chet I. Trichoderma — application, mode of action and potential as a biocontrol agent of soilborne plant pathogenic fungi. In: Chet I, ed. Innovative approaches to plant disease control. New York: John Wiley & Sons, 1987: 137–60.
Chet I, Baker R. Induction of suppressiveness to Rhizoctonia solani in soil. Phytopath 1980; 70: 994–8.
Cook RJ. Biological control of plant pathogens: Theory to application. Phytopath 1985; 75: 25–9.
Cook RJ, Baker KF. The nature and practice of biological control of plant pathogens. Minnesota: Amer Phytopathol Soc, 1983: 539 pp.
Dekker J, Georgopolous SG. Fungicide resistance in crop protection. Wageningen: Pudoc, 1982: 265 pp.
Dennis L, Webster J. Antagonistic properties of species — groups of Trichoderma. I. Production of non-volatile antibiotics. Trans Br mycol Soc 1971; 57: 25–39.
Dennis L, Webster J. Antagonistic properties of species — groups of Trichoderma. III Hyphal interaction. Trans Br mycol Soc 1971; 57: 363–9.
Elad Y, Misaghi IJ. Biochemical aspects of plant-microbe and microbe-microbe interaction in soil. In: CooperDriver GA, Swain T, eds. Chemically mediated interactions between plants and other organisms. New York: Plenum Press, 1985: 21–46.
Elad Y, Chet I, Henis Y. Degradation of plant pathogenic fungi by Trichoderma harzianum. Canad J Microbiol 1982; 28: 719–25.
Elad Y, Chet I, Boyle P, Henis Y. Parasitism of Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii. Scanning electron microscopy and fluorescence microscopy. Phytopath 1983; 28: 719–25.
Henis Y, Lewis JA, Papavizas GC. Interactions between Sclerotium rolfsii and Trichoderma spp. Relationship between antagonism and disease control. Soil Biol Biochem 1984; 16: 391–5.
Howell CR. Relevance of mycoparasitism in the biological control of Rhizoctonia solani by Gliocladium virens. Phytopath 1987; 77: 992–4.
Howell CR, Stipanovic RD. Gliovirin, a new antibiotic from Gliocladium virens, and its role in the biological control of Pythium ultimum. Canad J Microbiol 1983; 29: 321–4.
Huang HC. Gliocladium catenulatum: Hyperparasite of Sclerotinia sclerotiorum and Fusarium spp. Canad J Bot 1978; 56: 2243–6.
Lifshitz R, Windham MT, Baker R. Mechanism of biological control of preemergence damping-off of pea by seed treatment with Trichoderma spp. Phytopath 1986; 76: 720–5.
Lim TK, Chan LG. Parasitism of Phytophthora palmivora by Gliocladium roseum. Z Pflkr Plfschutz 1986; 93: 509–14.
Manibhushanrao K, Sreenivasaprasad S, Baby UI, Joe Y. Susceptibility of rice sheath blight pathogen to mycoparasites. Curr Sci 1989; 58: 515–8.
Okon Y, Chet I, Henis Y. Effect of lactose, ethanol and cycloheximide on the translocation pattern of radioactive compounds and of sclerotium formation in Sclerotium rolfsii. J gen Microbiol 1973; 74: 251–8.
Ooka T, Shimojima Y, Akimoto T, Takeda I, Senoh S, Abe J. A new antibacterial peptide ‘Suzukacillin’. Agri Biol Chem 1966; 30: 700–2.
Pachenari A, Dix NJ. Production of toxins and wall degrading enzymes by Gliocladium roseum. Trans Br mycol Soc 1980; 74: 561–6.
Papavizas GC. Trichoderma and Gliocladium: biology, ecology and potential for biocontrol. Ann Rev Phytopathol 1985; 23: 23–54.
Phillips AJL. Factors affecting the parasitic activity of Gliocladium virens on sclerotia of Sclerotinia sclerotiorum and a note on its host range. Phytopath Z 1986; 116: 212–20.
Sreenivasaprasad S. Studies on the hyperparasitic and biocontrol potential of Gliocladium virens and Trichoderma longibrachiatum. Ph D thesis Univ of Madras, India.
Tu JC. Gliocladium virens, a destructive mycoparasite of Sclerotinia sclerotiorum. Phytopath 1980; 70: 670–4.
Tu JC, Vaartaja O. The effect of hyperparasite (Gliocladium virens) on Rhizoctonia solani and Rhizoctonia root rot of white beans. Canad J Bot 1981; 59: 22–7.
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Sreenivasaprasad, S., Manibhushanrao, K. Antagonistic potential of Gliocladium virens and Trichoderma longibrachiatum to phytopathogenic fungi. Mycopathologia 109, 19–26 (1990). https://doi.org/10.1007/BF00437002
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DOI: https://doi.org/10.1007/BF00437002