Summary
Two new, recessive, chromosomal mutants of Neurospora, uvs-3, and uvs-4, have been found to be more sensitive to UV than wild type. The uvs-3 mutant, is very sensitive to nitrogen mustard, nitrosoguanidine and X-rays. UV-dose-response curves of uvs-3 lack the shoulder typical of wild type but parallel the wild-type curve at low survival levels. Unlike wild type, which shows a decrease in survival when incubated in water or proflavine (4 mg/ml) after UV, uvs-3 shows an increase in survival after water incubation and an even greater increase when incubated in proflavine. It also differs from wild type in showing much less photoreactivation and in not losing its photoreactivation ability during two hours incubation in water.
The uvs-4 mutant is not sensitive to nitrogen mustard and only slightly sensitive to nitrosoguanidine. Dose-response curves of uvs-4 retain a shoulder and are steeper than those of wild type at low survival levels. Proflavine sensitivity and photoreactivation of uvs-4 resemble wild type, but no decrease in survival occurs when it is held in water after UV.
The combination of characteristics shon, by uvs-4 is not like that of any of the UV-sensitive mutants found in E. coli but those of uvs-3 resemble those shown by the EXR (X-ray sensitive, low to normal recombination) and reckless REC (X-ray sensitive, recombinationless) mutant classes in bacteria.
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Communicated by E. Witkin
A portion of a dissertation submitted to the Graduate Division of Stanford University in partial fulfillment of the requirements for the degree of Doctor of Philosophy. This work was supported by a National Science Foundation predoctoral fellowship and Public Health Service Research grant AI-01462.
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Schroeder, A.L. Ultraviolet-sensitive mutants of Neurospora . Molec. Gen. Genet. 107, 305–320 (1970). https://doi.org/10.1007/BF00441193
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DOI: https://doi.org/10.1007/BF00441193