Summary
Two 30s ribosomal protein components, 30-4K and 30-7K, from E. coli K12 strain were clearly distinguished on the CMC column chromatogram from the corresponding protein components, 30-4B and 30-7B, from B strain. The 30-7K component was shown to correspond to the K-character.
A mutant strain of K12, W3637, had an altered 30s ribosomal protein component, 30-9W3637.
The characters of 30-4K, 30-7K, 30-9W3637 and str r were found to be cotransduced from W3637 to B strain by Plke phage in 16 out of 20 str r transductants. The 30-9W3637 and 30-4K components were separated from str r in 4 str r transductants. These results indicate that (1) neither 30-4 nor 30-9 is the protein whose mutational change leads to str r, and (2) the genes specifying the 30s ribosomal proteins, 30-4, 30-7, 30-9 and str are linked on the chromosome.
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Abbreviations
- CMC:
-
carboxymethyl cellulose
- str :
-
streptomycin
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Communicated by H. G. Wittmann
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Takata, R., Dekio, S., Otaka, E. et al. Genetic studies of the ribosomal proteins in Escherichia coli . Molec. Gen. Genet. 105, 113–121 (1969). https://doi.org/10.1007/BF00445680
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DOI: https://doi.org/10.1007/BF00445680