Abstract
(1) The active uptake of different amino acids by growing cells of Streptomyces hydrogenans was shown to be correlated with the physiological age of the cells. During the lag phase of growth the transport capacity increased and attained its highest level when the growth rate was maximum. During further growth the transport capacity declined progressively. The lowest transport activity was observed when the culture shifted into the stationary growth phase. (2) Such modulation of transport capacity was independent on the presence or absence of amino acids in the growth medium of the cells. (3) The size and the composition of the pool of free intracellular amino acids was also undergoing substantial variations during the growth cycle of the culture. In the lag phase, the levels of all amino acids decreased markedly and attained their lowest values at the end of this phase. During further growth the pool size was slowly replenished. (4) Removal of the pool resulted in a considerable gain of transport capacity. Therefore, it was concluded that active amino acid transport in growing Streptomyces hydrogenans is under feedback control by intracellular amino acids. (5) Quantitatively, the modulation of the pool size could not fully account for the variation of the transport capacity. Since a pool-independent stimulation of transport was found to be correlated with the increase of the growth rate of the cells, the possibility is discussed that the stimulation of transport is either due to increased levels of distinct RNA species, which might provide positive feedback signals for transport, or by increased rates of de novo synthesis of transport limiting proteins.
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Abbreviations
- AIB:
-
2-aminoisobutyric acid
- CM:
-
complete medium
- MM:
-
mineral medium
References
Benko, P. V., Wood, T. C., Segel, I. H.: Specificity and regulation of methionine transport in filamentous fungi. Arch. Biochem. Biophys. 122, 783–804 (1967)
Crabeel, M., Grenson, M.: Regulation of histidine uptake by specific feedback inhibition of two histidine permeases in Saccharomyces cerevisiae. Eur. J. Biochem. 14, 197–204 (1971)
Gross, W., Burkhardt, K. L.: Multiple transport systems for basic amino acid transport in Streptomyces hydrogenans. Biochim. biophys. Acta (Amst.) 298, 437–445 (1973)
Gross, W., Ring, K.: Effect of chloramphenicol on active amino acid transport. FEBS-Letters 4, 319–322 (1969)
Gross, W., Ring, K.: Active transport of glutamate in Streptomyces hydrogenans. Biochim. biophys. Acta (Amst.) 233, 652–665 (1971)
Gross, W., Ring, K., Heinz, E.: Positive feedback regulation of amino acid transport in Streptomyces hydrogenans. Arch. Biochem. Biophys. 137, 253–261 (1970)
Holden, J.: Amino acid pools. Amsterdam: Elsevier 1962
Kotyk, A.: Transport of α-aminoisobutyric acid in Saccharomyces cerevisiae. Feedback control. Biochim. biophys. Acta (Amst.) 288, 380–389 (1972)
LangHeinrich, W.: Untersuchungen über den Aminosäurepool und aktiven Aminosäuretransport bei Streptomyces hydrogenans. Thesis, Univ. Frankfurt (1972)
Maaløe, O., Kjeldgaard, N. O.: Control of macromolecular synthesis. New York: Benjamin 1966
Ogilvie, A., Wiebauer, K., Kersten, W.: Inhibition of leucyl-transfer ribonucleic acid synthetase in Bacillus subtilis by granaticin. Biochem. J. 152, 511–515 (1975)
Oxender, D.: Amino acid transport in microorganisms. In: Metabolic pathways, Vol. VI (L. E. Hokin, ed.), pp. 133–185. New York, Academic Press 1972
Pall, M. L., Kelly, K. A.: Specificity of transinhibition of amino acid transport in Neurospora. Biochem. biophys. Res. Commun. 42, 940–947 (1971)
Quay, St. C., Kline, E. L., Oxender, D. L.: Role of leucyl-tRNA synthetase in regulation of branched-chain amino acid transport. Proc. nat. Acad. Sci. (Wash.) 72, 3921–3924 (1975)
Ring, K.: The effect of low temperatures on permeability in Streptomyces hydrogenans. Biochem. biophys. Res. Commun. 19, 576–581 (1965)
Ring, K.: Die Induktion des aktiven Transportes neutraler Aminosäuren bei Streptomyces hydrogenans. Biochim. biophys. Acta (Amst.) 183, 375–393 (1969)
Ring, K.: Some aspects of active amino acid transport. Angew. Chemie (Intern. Edition) 9, 345–356 (1970)
Ring, K., Ehle, H., Foit, B.: Effect of alkali ions on the active transport of neutral amino acids into Streptomyces hydrogenans. Biochim. biophys. Acta (Amst.) 433, 615–629 (1976)
Ring, K., Gross, W., Heinz, E.: Negative feedback regulation of amino acid transport in Streptomyces hydrogenans. Arch. Biochem. Biophys. 137, 243–252 (1970)
Ring, K., Heinz, E.: Active amino acid transport in Streptomyces hydrogenans. Biochem. Z. 344, 446–461 (1966)
Rudorf, J. E.: Kinetische Untersuchungen zur Spezifität des aktiven Transportes neutraler Aminosäuren bei Streptomyces hydrogenans. Thesis, Univ. Frankfurt (1971)
Tempest, D. W., Meers, J. L., Brown, C. M.: Influence of environment on the content and composition of microbial free amino acid pools. J. gen. Microbiol. 64, 171–185 (1970)
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Langheinrich, W., Ring, K. Regulation of amino acid transport in growing cells of Streptomyces hydrogenans . Arch. Microbiol. 109, 227–235 (1976). https://doi.org/10.1007/BF00446633
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DOI: https://doi.org/10.1007/BF00446633