Summary
Gene conversion and postmeiotic segregation patterns have been analysed at 14 mutant sites of sup3, sup8 and sup9 including 5 alleles with a strong marker effect on recombination frequencies in two-factor crosses. The total frequency of gene conversion and postmeiotic segregation tetrads is fairly constant within each gene, but may vary from one gene to another. About 97% of the conversion events are coconversions spanning the whole sup gene. Postmeiotic segregations are usually quite rare. None of the marker-effect alleles has an increased rate of hybrid DNA formation at the allele considered, as judged from the frequency of gene conversion and postmeiotic segregation in one-factor crosses. At least two of them, sup3-e and sup9-e, are associated with a high frequency of postmeiotic segregation indicating a poor repair of the corresponding base-pair mismatches. This is also observed in a two-factor cross and can account for the marker effect on recombination frequencies. The properties of a third marker effect allele, sup3-e,r10, are best explained by a higher probability of single site conversions as opposed to coconversions in two-factor crosses involving the mutant site r10.
Similar content being viewed by others
References
Angehrn, P.: Untersuchungen über intragene Rekombinationsmechanismen und allele Komplementierung an Adeninmutanten von Schizosaccharomyces pombe. Ph. D. thesis, University of Zurich (1964)
Catcheside, D. G.: The genetics of recombination, 172pp. London: Edward Arnold Ltd. 1977
Drapeau, G. R., Brammar, W. J., Yanofsky, C.: J. Mol. Biol. 35, 357–367 (1967)
Fincham, J. R. S., Holliday, R.: Mol. Gen. Genet. 109, 309–322 (1970)
Goldman, S. L.: Mol. Gen. Genet. 132, 347–361 (1974)
Grossenbacher, A. M.: Untersuchungen zur spontanen mitotischen Rekombination im ade6 Gen von Schizosaccharomyces pombe. Diploma Thesis, University of Bern (1976)
Gutz, H.: Genetics 69, 317–337 (1971)
Gutz, H., Heslot, H., Leupold, U., Loprieno, N.: Schizosaccharomyces pombe. In: Handbook of Genetics. King, R. C. (ed.), Vol. 1, p. 395–446. New York and London: Plenum Press 1974
Hawthorne, D. C., Leupold, U.: Curr. Top. Microbiol. Immunol. 64, 1–47 (1974)
Hofer, F., Hollenstein, H., Janner, F., Minet, M., Thuriaux, P., Leupold, U.: Current Genet. 1, 45–61 (1979)
Kohli, J., Hottinger, H., Munz, P., Strauss, A., Thuriaux, P.: Genetics 87, 471–489 (1977)
Kohli, J., Altruda, F., Kwong, T., Rafalski, A., Wetzel, R., Still, D., Wahl, G., Leupold, U.: Nonsense suppressor transfer RNAs in Schizosaccharomyces pombe. In: Transfer RNA. Abelson, J., Schimmel, P., Söll, D. (eds.), New York: Cold Spring Harbor Laboratory, in press (1979a)
Kohli, J., Kwong, T., Altruda, F., Still, D., Wahl, G.: J. Biol. Chem. 254, 1546–1551 (1979b)
Leblon, G., Rossignol, J.-L.: Mol. Gen. Genet. 122, 165–182 (1973)
Leupold, U.: Cold Spring Harbor Symp. Quant. Biol. 23, 161–170 (1958)
Moore, C., Sherman, F.: Genetics 85, 1–22 (1977)
Munz, P., Leupold, U.: Mol. Gen. Genet. 170, 145–148 (1979)
Norkin, L. C.:Mol. Biol. 51, 633–655 (1971)
Nurse, P., Thuriaux, P.: Exp. Cell Res. 107 365–375 (1977)
Pukkila, P. J.: Mol. Gen. Genet. 161, 245–250 (1978)
Rafalski, A., Kohli, J., Agris, P., Söll, D.: Nucl. Acids Res. 6, 2683–2695 (1979)
Rossignol, J. L., Haedens, V.: Heredity 40, 405–425 (1978)
Stahl, F. W., Craseman, J. M., Stahl, M. M.: Genetics 94, 203–212 (1975)
Thuriaux, P., Minet, M., Hofer, F., Leupold, U.: Mol. Gen. Genet. 142, 251–261 (1975)
Thuriaux, P.: Nature 268, 460–462 (1977)
Wetzel, R., Kohli, J., Altruda, F., Söll, D.: Mol. Gen. Genet. 172, 221–228 (1979)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thuriaux, P., Minet, M., Munz, P. et al. Gene conversion in nonsense suppressors of Schizosaccharomyces pombe . Curr Genet 1, 89–95 (1980). https://doi.org/10.1007/BF00446954
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00446954