Abstract
pIJ1008, a Rhizobium leguminosarum plasmid which determines hydrogen uptake ability and symbiotic functions in pea was transferable to three of seven natural isolates of R. meliloti tested. In these three strains, pIJ1008 was maintained stably with the respective sym megaplasmid indigenous to each R. meliloti strain. These strains carrying both plasmids nodulated alfalfa but not pea. By reisolation and examination of the strains from alfalfa nodule tissue, it was shown that pIJ1008 continued to be maintained but that pea-nodulation ability was suppressed.
In one strain of R. meliloti which carries a 200 kb cryptic plasmid (in addition to a megaplasmid), the transfer and selection for pIJ1008 resulted in the loss of the cryptic plasmid.
In three separate plant growth experiments, alfalfa nodules induced by each of the R. meliloti strain carrying both sym plasmids were assayed for hydrogen uptake activity. The average activity was 40-, 3.5-and 2-fold higher than with the respective pIJ1008-free strains. However, this higher activity was not accompanied by an increase in plant biomass or nitrogen content of shoots.
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Alorocht SL, Maier RJ, Hanus JF, Russel SA, Emerich DW, Evans HJ (1979) Hydrogenase in Rhizobium japonicum increases nitrogen fixation by nodulated soybeans. Science 203:1255–1257
Banfalvi Z, Sakanyan V, Koncz C, Kiss A, Dusha I, Kondorosi A (1981) Location of nodulation and nitrogen fixation genes on a hight molecular weight plasmid of Rhizobium meliloti. Mol Gen Genet 184:318–325
Bedmar EJ, Edie SA, Phillips DA (1983) Host plant cultivar effects on hydrogen evolution by Rhizobium leguminosarum. Plant Physiol 72:1011–1015
Bedmar EJ, Brewin NJ, Phillips DA (1984) Effect of plasmid pIJ1008 from Rhizobium leguminosarum on symbiotic function of Rhizobium meliloti. Appl Environ Microbiol 47:876–878
Beringer JE (1974) R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 84:1255–1257
Bethlenfalvay GJ, Phillips DA (1979) Variation in nitrogenase and hydrogenase activity of Alaska pea root nodules. Plant Physiol 63:816–820
Brewin NJ, De Jong TM, Phillips DA, Johnston AWB (1980) Cotransfer of determinants for hydrogenase activity and nodulation ability in Rhizobium leguminosarum. Nature 288:77–79
Brewin NJ, Wood EA, Johnston AWB, Dibb NJ, Hombrecher G (1982) Recombinant nodulation plasmids in Rhizobium leguminosarum. J Gen Microbiol 128:1817–1827
Carter KR, Jennings NT, Hanus J, Evans HJ (1978) Hydrogen evolution and uptake by nodules of soybeans inoculated with different strains of Rhizobium japonicum. Can J Microbiol 21:307–311
DeJong TM, Brewin NJ, Johnston AWB, Phillips DA (1982) Improvement of symbiotic properties in Rhizobium leguminosarum by plasmid transfer. J Gen Microbiol 128:1829–1838
Dixon ROD (1972) Hydrogenase in legume root nodule bacteroids: occurrence and properties. Arch Mikrobiol 85:193–201
Eckhardt T (1978) A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1:584–588
Eisbrenner G, Evans HJ (1983) Aspects of hydrogen metabolism in nitrogen-fixing legumes and other plant-microbe associations. Ann Rev Plant Physiol 34:105–136
Emerich DW, Ruiz-Argueso T, Ching TM, Evans HJ (1979) Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids. J Bacteriol 137:153–160
Hooykaas PJJ, Snijdewint FGM, Schilperoort RA (1982) Identification of Sym plasmid of Rhizobium leguminosarum strain 1001 and its transfer to and expression in other Rhizobia and Agrobacterium tumefaciens. Plasmid 8:73–82
Johnston AWB, Setchell SM, Beringer JE (1978) Interspecific crosses between Rhizobium leguminosarum and R. meliloti: formation of haploid recombinants and of R-primes. J Gen Microbiol 104:209–218
Lepo JE, Hickok RE, Cantrell MA, Russel SA, Evans HJ (1981) Revertible hydrogen uptake deficient mutants of Rhizobium japonicum. J Bacteriol 146:614–620
Lim ST (1978) Determination of hydrogenase in free-living cultures of Rhizobium japonicum and energy efficiency of soybean nodules. Plant Physiol 62:609–611
Miller RW, Sirois JC (1982) Relative efficacy of different alfalfa cultivar-Rhizobium meliloti combinations for symbiotic nitrogen fixation. Appl Environ Microbiol 43:764–768
Nelson BM, Child JJ (1981) Nitrogen fixation and hydrogen metabolism by Rhizobium leguminosarum isolates in pea root nodules. Can J Microbiol 27:1028–1034
Nelson BM, Salmine SO (1982) Uptake hydrogenase activity and ATP formation in Rhizobium leguminosarum bacteroids. J Bacteriol 151:989–995
Putnoky P, Kiss GB, Ott I, Kondorosi A (1983) Tn5 carries a streptomycin-resistance determinant downstream from the kanamycin-resistance gene. Mol Gen Genet 191:288–294
Rosenberg C, Botstard P, Denarie J, Casse-Belbart F (1981) Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti. Mol Gen Genet 184:326–333
Ruiz-Argueso T, Hanus FJ, Evans HJ (1978) Hydrogen production and uptake by pea nodules as affected by strains of Rhizobium leguminosarum. Arch Microbiol 116:113–118
Ruiz-Argueso T, Maier RJ, Evans HJ (1979) Hydrogen evolution from alfalfa and clover nodules and hydrogen uptake by free living Rhizobium meliloti. Appl Environ Microbiol 37:582–587
Schubert KR, Evans HJ (1976) Hydrogen evolution: a major factor affecting the efficiency of nitrogen fixation in nodulated symbionts. Proc Natl Acad Sci USA 73:1207–1211
Selvaraj G, Iyer VN (1984) Transposon Tn5 specifies streptomycin resistance in Rhizobium spp. J Bacteriol 158:580–589
Vincent JM (1970) A manual for the practical study of root nodule bacteria. IBP Handbook No. 15. Blackwell Scientific Publication, Oxford
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C.B.R.I. Contribution Number: 1478
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Behki, R.M., Selvaraj, G. & Iyer, V.N. Derivatives of Rhizobium meliloti strains carrying a plasmid of Rhizobium leguminosarum specifying hydrogen uptake and pea-specific symbiotic functions. Arch. Microbiol. 140, 352–357 (1985). https://doi.org/10.1007/BF00446977
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DOI: https://doi.org/10.1007/BF00446977