Abstract
The spectroscopically different phycocyanins present in the type strain of Hemiselmis virescens, Millport 64, and in a second strain of this cryptophytan species, Plymouth 157, have been purified and compared. They are similar in native molecular weight and in subunit structure, both containing α and β subunits with molecular weights of approximately 10 000 and 19 000 respectively. However, they do not have the same chromophore composition. Both subunits of the phycocyanin of Plymouth 157 contain two bilins, with absorption maxima at 600 and 660 nm, respectively. Only the β subunit of Millport 64 carries these chromophores; its α subunit bears another chromophore, with absorption maxima at 368 and 694 nm. The spectroscopic differences between the two native phycocyanins can be entirely accounted for by their differing chromophore compositions. The phycocyanin of Millport 64 is the only biliprotein so far described which contains three chemically different chromophores.
Similar content being viewed by others
References
Allen, M. B., Dougherty, E. C., McLaughlin., J. J. A.: Chromoprotein of some cryptomonad flagellates. Nature (Lond.) 184, 1047–1049 (1959)
Antia, N. J., Chorney, V.: Nature of the nitrogen compounds supporting phototrophic growth of the marine cryptomonad Hemiselmis virescens. J. Protozool. 15, 198–201 (1968)
Brooks, C., Gantt, E.: Comparison of phycoerythrins (542, 566 nm) from cryptophycean algae. Arch. Mikrobiol. 88, 193–204 (1973)
Chapman, D. J., Cole, W. J., Siegelman, H. W.: Chromophores of allophycocyanin and R-phycocyanin. Biochem. J. 105, 903–905 (1967)
Glazer, A. N., Cohen-Bazire, G., Stanier, R. Y.: Characterization of phycoerythrin from a Cryptomonas sp. Arch. Mikrobiol. 80, 1–18 (1971)
Glazer, A. N., Fang, S.: Formation, of hybrid proteins from the α and β subunits of phycocyanins of unicellular and filamentous blue-green algae. J. biol. Chem. 248, 663–671 (1973)
MacColl, R., Habig, W., Berns, D. S.: Characterization of phycocyanin from Chroomonas species. J. biol. Chem. 248, 7080–7086 (1973)
O'Carra, P.: Algal biliproteins. Biochem. J. 119, 2P-3P (1970)
O'hEocha, C., O'Carra, P., Mitchell, D.: Biliproteins of cryptomonad algae. Proc. roy. Irish Acad. B. 63, 191–200 (1964)
O'hEocha, C., Raftery, M.: Phycoerthrins and phycocyanins of cryptomonads. Nature (Lond.) 184, 1049–1051 (1959)
Provasoli, L., McLaughlin, J. J. A., Droop, M. R.: The development of artificial, media for marine algae. Arch. Mikrobiol. 25, 392–428 (1957)
Weber, K., Osborn, M.: The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. biol. Chem. 244, 4406–4412 (1969)
Wrigley, C.: Gel electrofocusing—A technique for analyzing multiple protein samples by isoelectric focusing. Sci. Tools 15, 17–23 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Glazer, A.N., Cohen-Bazire, G. A comparison of cryptophytan phycocyanins. Arch. Microbiol. 104, 29–32 (1975). https://doi.org/10.1007/BF00447296
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00447296