Summary
Histone and non-histone-proteins (NHP) are proteins with a specific affinity to DNA; each group is involved in the regulation of gene expression in its own way. To investigate molecular biologic processes at psoriasis, DNA-binding proteins (DBP) were isolated from psoriatic scales. During this work the proportions of histone and NHP of the DBP fractions were examined. Using two different methods (Bio Rex 70-chromatography and isoelectrofocussing), it was found that the isolated DBP contain only acidic proteins. Likewise, the composition of amino acids is comparable with those of acidic, nuclear proteins of other tissues. Thus the isolated DBP represent to its greatest extent acidic, chromosomal NHP, which obviously are derived from the preserved nuclei of the parakeratotic scale layer.
Zusammenfassung
Histone und Nicht-Histon-Protein (NHP) sind Proteine mit spezifischer DNA-Affinität; jede Gruppe besitzt eine eigene Funktion bei der Regulation von Genaktivitäten. Um die Pathogenese der Psoriasis auf molekularbiologischer Ebene untersuchen zu können, wurden DNA-affine Proteine (DBP) aus Psoriasisschuppen isoliert. Im Rahmen dieser Arbeit sollte der Anteil an Histonen und NHP in den isolierten DBP festgestellt werden. Mit zwei verschiedenen Methoden (Bio-Rex 70-Chromatographie, Isoelektrofokussierung) wurde jedoch nachgewiesen, daß die isolierten DBP nur saure Proteine enthalten. Auch die Zusammensetzung der Aminosäuren ist mit der von sauren Kernproteinen anderer Gewebe vergleichbar. Somit stellen die isolierten DBP weitestgehend saure chromosomale NHP dar, die offensichtlich den erhaltenen Kernen der parakeratotischen Schuppenschicht entstammen.
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Supported by grant of the Deutsche Forschungsgemeinschaft
We are greatly indebted Mrs. M. Mentzer for her competent assistance
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Reimer, G., Leonhardi, G. & Schwulera, U. DNA-binding proteins of psoriatic scales. Arch Dermatol Res 262, 185–189 (1978). https://doi.org/10.1007/BF00455389
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DOI: https://doi.org/10.1007/BF00455389