Abstract
By means of the zymogram technique, two types of electrophoretic patterns were found for the enzyme alcohol dehydrogenase (ADH) in Triticum dicoccum, a tetraploid species of wheat. Each strain examined showed three bands of ADH activity. The two types of patterns found differed with respect to the relative electrophoretic mobilities and staining intensities of the bands. Evidence that the variation between the patterns is controlled at a single gene locus by two codominant alleles was obtained from appropriate genetic crosses. Heterozygotes for the allelic difference have five bands of ADH activity. These probably represent six different forms of the enzyme, two of which have coincident electrophoretic mobilities. These observations support the hypothesis that ADH exists as a dimer in T. dicoccum. It is probable that the enzyme subunits are coded for by duplicated ADH gene loci, each of which was contributed to the original tetraploid wheats by one of the two diploid progenitors of that group.
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This paper is Technical Article No. 7983, Texas Agricultural Experiment Station.
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Hart, G.E. Genetic control of alcohol dehydrogenase isozymes in Triticum dicoccum . Biochem Genet 3, 617–625 (1969). https://doi.org/10.1007/BF00485484
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DOI: https://doi.org/10.1007/BF00485484