Abstract
A study of aldehyde dehydrogenase in rat hepatoma cells and rat hepatoma-mouse fibroblast hybrids revealed that the hepatoma cells had activity comparable to that found in whole rat liver and that the enzyme activity was suppressed in early hybrids and reappeared following chromosome loss. Starch gel electrophoresis and heat inactivation studies showed that a new form of enzyme was produced in the hybrids, possibly a heteropolymorphic combination between the HTC enzyme and a previously repressed mouse form. Staining methods for starch gel electrophoresis and histochemical detection of aldehyde dehydrogenase are described.
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This work was supported by grants from the Damon Runyon Foundation (DRG 1088s) and the Public Health Service (1-R01-Ca 12310-02).
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Rintoul, D., Lewis, R.F. & Morrow, J. Aldehyde dehydrogenase isozymes in rat hepatoma-mouse fibroblast hybrids. Biochem Genet 9, 375–387 (1973). https://doi.org/10.1007/BF00486073
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DOI: https://doi.org/10.1007/BF00486073