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Enzyme histochemical observations on the segmentation of the proximal tubules in kidney of the female rat

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The segmentation of the proximal tubules in the kidney of the female rat was studied by means of enzyme histochemical reactions and the results compared with those observed in the male and recently described by Jacobsen and Jørgensen (1973a). Reactions were performed for the following soluble, coenzyme-dependent oxido-reductases: glucose 6-phosphate dehydrogenase, α-glycerophosphate dehydrogenase, 3 α-hydroxysteroid dehydrogenase, NAD-as well as NADP-dependent isocitrate dehydrogenases, NAD-dependent malate dehydrogenase, NADP-dependent, decarboxylating malate dehydrogenase, uridine diphosphate glucose dehydrogenase. Measures were taken to reduce enzyme diffusion and eliminate interference from tissue tetrazolium reductases. Furthermore, reactions were performed for a number of less soluble or insoluble enzymes: glucose 6-phosphatase, mitochondrial α-glycerophosphate dehydrogenase, β-hydroxybutyrate dehydrogenase, succinate dehydrogenase and tetrazolium reductases.

In the proximal tubules of the female rat all enzymes studied—except β-hydroxybutyrate dehydrogenase—showed segmental differences, most of them clearly revealing three segments. Sex differences were found concerning all enzymes except uridine diphosphate glucose dehydrogenase and NADP-dependent isocitrate dehydrogenase. The most pronounced sex-related differences were seen in the third segment in which part the male rat showed highest activity in respect to tetrazolium reductases, NAD-dependent isocitrate dehydrogenase, succinate dehydrogenase, β-hydroxybutyrate dehydrogenase, 3 α-hydroxysteroid dehydrogenase and glucose 6-phosphate dehydrogenase and the female in respect to glucose 6-phosphatase, α-glycerophosphate dehydrogenases, and NADP-dependent, decarboxylating malate dehydrogenase. A few of the enzymes exhibited minor sex differences in the first two segments.

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    N. O. Jacobsen

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Supported by grants from Fonden til Lægevidenskabens Fremme and the Danish Medical Research Council.—Mrs. M. Jacobsen is thanked for excellent technical assistance.

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Jacobsen, N.O. Enzyme histochemical observations on the segmentation of the proximal tubules in kidney of the female rat. Histochemistry 43, 11–32 (1975). https://doi.org/10.1007/BF00490151

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