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Ultrastructural study of the distribution of calcium in the pineal gland of the rat subjected to manipulation of the photoperiod

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Summary

Using the pyroantimoniate technique, a study was conducted at electron microscope level on the distribution of the calcium ion in the pineal glands of normal adult Sprague-Dawley rats with initial weights of 150–200 g subjected to a 12:12 light dark cycle and others under the same conditions were subjected to modifications in the noradrenergic signal, such as continuous illumination over 7 days, blinding by bilateral enucleation (7 or 90 days) before sacrifice and bilateral superior cervical gangliectomy at 21 days before sacrifice. All the animals were sacrificed by decapitation, half of them at midday and the other half at midnight. Abundant fine precipitations of calcium were found in the intercellular spaces of the pineal glands of the normal rats. By contrast, in the gangliectomized animals subjected to constant illumination and chronic binding these precipitations were few in number. Additionally, two types of pinealocytes were observed regarding the distribution and concentration of intracytoplasmic calcium in both the normal and experimentally manipulated animals. Type I correspond to the classic light pinealocytes, with an absence of intracytoplasmic precipitations, although in the normal and gangliectomized animals sacrificed at midnight it was possible to observe fine deposits inside the mitochondrial matrix. Type II correspond to the classic dark pinealocytes, with a dense cytoplasmic matrix and numerous deposits of intracytoplasmic and intranuclear calcium; these were never seen in the type I pinealocytes.

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References

  • Allen DJ, Allen JS, Didio LJA, McGrath JA (1981) Scanning electron microscopy and X-ray microanalysis of the human pineal body with emphasis on calcareous concretions. J Submicrosc Cytol 13:675–695

    Google Scholar 

  • Appleton J, Morris DC (1979) The use of the potassium pyroantimonate osmium method as a means of identifying and localizaing calcium at the ultrastructural level in the cells of calcifying systems. J Histochem Cytochem 27:676–680

    Google Scholar 

  • Arstila AU (1967) Electron microscopic studies on the structure and histochemistry of the pineal gland of the rat. Neuroendocrinology 2 (Suppl):1–101

    Google Scholar 

  • Arstila AV, Hospsu VK (1964) Studies in the rat pineal gland. I. Ultrastructure. Ann Acad Sci Fenn Ser A 3 113:3–21

    Google Scholar 

  • Brammer GL, Morley JE, Geller E, Yuwiler A, Hershman JM (1979) Hypothalamus-pituitary-thyroid axis interactions with pineal gland in the rat. Am J Physiol 236:E416–420

    Google Scholar 

  • Buijs RM, Pevet P (1980) Vasopressin and oxytocin containing fibers in the pineal gland and subcommissural organ of the rat. Cell Tissue Res 205:11–17

    Google Scholar 

  • Del Rio Hortega P (1932) Pineal gland. In: Penfield W (ed) Cytology and cellular pathology of the nervous system, vol 2. Hoeber, New York, pp 637–703

    Google Scholar 

  • Diehl BJM (1978) Occurrence and regional distribution of calcarcus concretions in the rat pineal gland. Cell Tissue Res 195:359–366

    Google Scholar 

  • Eisenmann DR, Ashrafi S, Neiman A (1979) Calcium transport and the secretory ameloblast. Anat Rec 193:403–422

    Google Scholar 

  • Erding F (1977) Concrement formation encountered in the rat pineal gland. Experientia 33:514

    Google Scholar 

  • Fernstrom JD, Fisher LA, Cusak BM, Gillis MA (1980) Radioimmunologic detection and measurement of nonapeptides in the pineal gland. Endocrinology 106:243–251

    Google Scholar 

  • Gusek W (1976) Die Feinstruktur der Rattenzirbel und ihr Verhalten unter Einfluß von Antiandrogen und nach Kastration. Endocrinologie 67:129–151

    Google Scholar 

  • Gusek W, Buss H, Wartenberg H (1965) Weitere Untersuchungen zur Feinstruktur der Epiphysis cerebri normaler and vorbehandelter Ratten. Prog Brain Res 10:317–330

    Google Scholar 

  • Japah JL, Elder TJ, Goldsmith ED (1976) Calcified inclusions in the superficial pineal gland of the Mongolian gerbil Meriones unguiculatus. Acta Anat 94:533–544

    Google Scholar 

  • Joseph SA (1976) Seasonal variation and luteinizing hormone releasing hormone (LHRH) content of rat pineal gland. Anat Rec 134:439

    Google Scholar 

  • Karasek M (1971) The role of the pineal body in mammals Polish. Endocrinology 22:315–327

    Google Scholar 

  • Karasek M (1983) Ultrastructure of the mammalian pineal gland: its comparative and functional aspects. Pineal Res Rev 1: 1–48

    Google Scholar 

  • Kitay JI, Altschule MD (1954) The pineal gland: A review of the physiologic literature. Harvard University Press, Cambridge, MA

    Google Scholar 

  • Klein DC (1979) Circadian rhythms in the pineal gland. In: Krieger DT (ed.) Endocrine rhythms. Raven Press, New York, pp 203–224

    Google Scholar 

  • Klein DC, Weller JL (1972) Rapid light-induced decrease in pineal serotonin N-acetyltransferase activity. Science 177:532–533

    Google Scholar 

  • Klein DC, Vanecek J, Sugden D, Sugden AL, Chik CL, Ho AK (1987) The dual receptor regulation of the mammalian pinealocyte: integration of peptide and catecholamine input. IV Colloqium of the European Pineal Study Group, Modena, p 14

  • Krstie R (1985) Ultracytochemical localization of calcium in the superficial pineal gland of the Mongolian gerbil. J Pineal Res 2:21–37

    Google Scholar 

  • Krstic R (1986) Pineal calcification: Its mechanis and significance. J Neural Transm (Suppl) 21:415–432

    Google Scholar 

  • Leuschen MP, Morirarty CM, Sampson HW, Piscopo I (1981) Cytochemical analysis of intracellular calcium distribution in the anterior pituitary of the rat. Cell Tissue Res 220:191–200

    Google Scholar 

  • Lukaszyk A, Reiter RJ (1975) Histophysiological evidence for the secretion of polypeptides by the pineal gland. Am J Anat 143:451–464

    Google Scholar 

  • Mitcu SM, Pavel S, Neascu C (1963) Biological and chromatographic characterization of a polypeptide which pressor and oxytocic activities isolated from bovine pineal gland. Endocrinology 72:563–566

    Google Scholar 

  • Moore RY (1975 Indoleamine metabolism in the intact and denervated pineal, pineal stalk, and habenula. Neuroendocrinology 19:323

    Google Scholar 

  • Moriarty CM (1978) Role of calcium in the regulation of adenohypophysial hormone release. Life Sci 23:185–194

    Google Scholar 

  • Pevet P (1977) On the presence of different populations of pinealocytes in the mammalian pineal gland. J Neurol Transm 40:289–304

    Google Scholar 

  • Pevet P (1981) The Pineal Gland. In: Reiter RA (ed) Anatomy and biochemistry, vol 1. CRC Press, Boca Raton, Florida, pp 121–154

    Google Scholar 

  • Pizarro MDL, Gil JAL, Vasallo JL, Muñoz Barragan L (1988) Rat pineal gland calcium distribution. In: Proceedings of Symposium on Melatonin and The Pineal Gland. Chin J Physiol 4:267

  • Pohl CR, Gibbs FP (1978) Circadian rhythms in blinded rats: Correlation between pineal and activity cycles. Am J Physiol 234:R110-R114

    Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–213

    Google Scholar 

  • Satodate R, Katsura S, Ota M (1973) Structural Changes of the pineals of blinded rats fed long term. Experientia 29:1414–1415

    Google Scholar 

  • Scharenberg K, Liss L (1965) The histologic structure of the human pineal body. Prog Brain Res 10:193–217

    Google Scholar 

  • Sheridan MN, Reiter RJ (1968) The fine structure of the hamster pineal gland. Am J Anat 122:357–376

    Google Scholar 

  • Somlyo AP (1984) Cellular site of calcium regulation. Nature 309:516–517

    Google Scholar 

  • Sugden AL, Sugden D, Klein DC (1986) Essential role of calcium influx in the adrenergic regulation of CAMP and C GMP in rat pinealocytes. J Biol Chem 261:11608–11612

    Google Scholar 

  • Sugden AL, Sugden D, Klein DC (1987) 169-1 activation elevates cytosolic calcium in rat pinealocytes by increasing net influx. J Biol Chem 262:741–745

    Google Scholar 

  • Tapp E, Huxley M (1971) The weight and degree of calcification of the pineal gland. J Pathol 105:31–39

    Google Scholar 

  • Tapp E, Huxley M (1972) The histological appearance of the human pineal gland from puberty to old age. J Pathol 108:137–144

    Google Scholar 

  • Trentini GP, Gaetani CF, Pierini G, Criscuolo M, Vidyasagar RI, Fabbri F (1986) Some aspects of human pineal pathology. In: Reiter RJ, Karasek M (eds) Advances in pineal research, vol 1. John Libbey, London, pp 219–229

    Google Scholar 

  • Upson RH, Benson B, Satterfield V (1976) Quantitation of ultrastructural changes in the mouse pineal in response to continuous illumination. Anat Rec 184:311–324

    Google Scholar 

  • Waltz B (1982) Ca2+ sequestering smooth endoplasmic reticulum in an invertebrate photoreceptor. I. Intracellular topography as revealed by OsFeCN staining and in situ accumulation. J Cell Biol 93:839–848

    Google Scholar 

  • Weiss B, Costa E (1967) Adenyl cyclase activity in rat pineal gland: effects of chronic denervation and norepinephrine. Science 156:1750–1752

    Google Scholar 

  • Welsh MG (1984) Cytochemical analysis of calcium distribution in the superficial pineal gland of the Mongolian gerbil. J Pineal Res 1:305–316

    Google Scholar 

  • Welsh MG (1985) Pineal calcification: Structural and functional aspects. Pineal Res Rev 3:41–68

    Google Scholar 

  • Welsh MG, Reiter RJ (1978) The pineal gland of the gerbil, Meriones unguiculatus. I. An ultrastructural study. Cell Tissue Res 193: 323–336

    Google Scholar 

  • Wick SM, Hepler PK (1982) Selective localization of intracellular Ca2+ with potassium antimonate. J Histochem Cytochem 30:1190–1204

    Google Scholar 

  • Zimmermann RA, Bilaniuk LT (1982) Age-related incidence of pineal calcification detected by computed tomography. Radiology 142:659–662

    Google Scholar 

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Authors and Affiliations

  1. Department of Investigation and Electron Microscopic, Hospital Clinico, Paseo de S. Vicente, s/n, E-37007, Salamanca, Spain

    M. D. L. Pizarro, F. E. Pastor, A. López Gil & L. Muñoz Barragán

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  1. M. D. L. Pizarro
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  2. F. E. Pastor
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  3. A. López Gil
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  4. L. Muñoz Barragán
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Pizarro, M.D.L., Pastor, F.E., Gil, A.L. et al. Ultrastructural study of the distribution of calcium in the pineal gland of the rat subjected to manipulation of the photoperiod. Histochemistry 92, 161–169 (1989). https://doi.org/10.1007/BF00490237

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  • DOI: https://doi.org/10.1007/BF00490237

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