Abstract
The Na level inside cells of Anacystis nidulans is lower than in the external medium reflecting an effective Na extrusion. Na efflux is an active process and is driven by a Na+/H+-antiport system. The necessary H+-gradient is generated by a proton translocating ATPase in the plasmalemma. This ATPase (electrogenic proton pump) also produces the membrane potential (about -110 mV) responsible for K accumulation. N,N′-dicyclohexylcarbodiimide (DCCD) inhibits the ATPase and the H+-gradient completely, but the membrane potential is only reduced (<-70 mV), since K efflux initiated by DCCD maintains the potential partly by diffusion potential.
With DCCD, active Na efflux is inhibited thus revealing Na uptake and leading by equilibration to the membrane potential to a 5–20 fold accumulation. Na uptake depends on the DCCD concentration with an optimum at (1–2)×10-4 M DCCD. Pretreatment with DCCD for a few minutes followed by replacement of the medium suffices to induce Na uptake.
DCCD induced Na influx is about 5 times faster in light than in darkness, and the steady state is reached much earlier in light; a 5 fold increase by light was also found for Rb uptake with untreated cells. Valinomycin stimulates the influx of Rb to about the same rate in light and dark. Therefore light may unspecifically increase the permeability of the plasma-lemma probably via the ATP level. Similarly to DCCD also 3×10-3 M N-ethylmaleimide induces Na uptake.
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Abbreviations
- DCCD:
-
N,N′-dicyclohexylcarbodiimide
- NEM:
-
N-ethylmaleimide
- CCCP:
-
carbonylcyanide m-chlorophenylhydrazone
- Pipes:
-
piperazine-N,N′-bis(2-ethanesulfonic acid)
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
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Paschinger, H. DCCD induced sodium uptake by Anacystis nidulans . Arch. Microbiol. 113, 285–291 (1977). https://doi.org/10.1007/BF00492037
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DOI: https://doi.org/10.1007/BF00492037