Abstract
Resting cell cultures of Aspergillus parasiticus were grown in medium containing four different concentrations of glucose, with and without acetone. In addition, the effect of different equimolar concentrations of acetone, acetic acid, ethanol, and sodium acetate was compared at two glucose levels. Aflatoxin and versicolorin pigment production increased in resting cell medium containing increasing concentrations of glucose. In the presence of glucose high concentrations of acetone (1.0 and 0.25 M) inhibited secondary biosynthesis and low concentrations of acetone (0.1, 0.025 and 0.01 M) stimulated secondary biosynthesis of aflatoxins and versicolorin pigments.
Similar content being viewed by others
References
Adye, J. & R. I. Matales. 1964. Incorporation of labelled compounds into aflatoxins. Biochem. Biophys. Acta 86: 418–420.
Basappa, S. C., A. Jayaraman, V. Screenivasamurthy & H. A. B. Parpia. 1967. Effect of B-group vitamins and ethyl alcohol on aflatoxin production by Aspergillus oryzae. Indian J. Exp. Biol. 5: 262–263.
Basappa, V. Screenivasamurthy & H. A. B. Parpia. 1970. Aflatoxin and kojic acid production by resting cells of Aspergillus flavus Link. J. Gen. Microbiol. 61: 81–86.
Demain, A. L. 1973. Mutation and the production of secondary metabolites. Adv. Appl. Microbiol. 16: 177–202.
Detroy, R. W., E. B. Lillehoj & A. Ciegler. 1971. Aflatoxin and related compounds. In: A. Ciegler, S. Kadis & S. J. Ajl (eds), ‘Microbial Toxins Vol. VI Fungal Toxins.’ Academic Press, N.Y. 4–178.
Gupta, S. R., H. R. Prasanna, L. Viswanathan & T. A. Venkitasubramanian. 1974. Carboxylic acid as carbon source for aflatoxin production. Experimentia 30: 1244–1246.
Heathcote, J. G., M. F. Dutton & J. R. Hibbert. 1973. Biosynthesis of aflatoxins. Chem and Ind. 21: 1027–1038.
Hsieh, D. P. H., M. T. Lin & R. C. Yao. 1973. Conversion of sterigmatocystin to aflatoxin B by Aspergillus parasiticus. Biochem. Biophys. Res. Comm. 52: 992–997.
Hsieh, D. P. H. & R. I. Matales. 1970. The relative contribution of acetate and glucose to aflatoxin biosynthesis. Biochem. Biophys. Acta: 208: 482–486.
Hsieh, D. P. H. & R. I. Matales. 1971. Preparation of labelled aflatoxins with high specific activities. Appl. Microbiol. 22: 79–83.
Lee, L. S., J. W. Bennett, A. F. Cucullu & R. L. Ory. 1975. Biosynthesis of aflatoxin B1: conversion of versicolorin A to aflatoxin B1 by Aspergillus parasiticus. Biochemistry (in preparation).
Lin, M. T., D. P. H. Hsieh, R. C. Yao & J. A. Donkersloot. 1973. Conversion of averufin into aflatoxins by Aspergillus parasiticus. Biochemistry 12: 5167–5171.
Lin, M. T. & D. P. H. Hsieh. 1973. Averufin in the biosynthesis of aflatoxin B1. J. Am. Chem. Soc. 95: 1668–1669.
Mayne, R. Y., J. W. Bennett & J. Tallant. 1971. Instability of an aflatoxin-producing strain of Aspergillus parasiticus. Mycologia 63: 644–648.
Moss, M. O. 1972. Aflatoxins and related mycotoxins. In: J. B. Harborne (ed.), Phytochemical Ecology, pp. 125–144. Academic Press, N.Y.
Pons, W. A., A. F. Cucullu, A. O. Franz & L. A. Goldblatt. 1968. Improved objective fluorodensitometric determination of aflatoxins in cottonseed products. J. Am. Oil Chem. Soc. 45: 694–699.
Roberts, J. C. 1974. Aflatoxins and sterigmatocystins. Fortschritte der chemie organischer Naturstoff 31: 119–151.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bennett, J.W., Lee, L.S. & Gaar, G.G. Effect of acetone on production of aflatoxins and versicolorin pigments by resting cell cultures of aspergillus parasiticus. Mycopathologia 58, 9–12 (1976). https://doi.org/10.1007/BF00493586
Issue Date:
DOI: https://doi.org/10.1007/BF00493586