Summary
Rabbits immunized with low-activity ruminal carbonic anhydrase (RCA) isoenzyme, extracted from ruminal epithelial cells isolated by digestion with trupsin, yielded anti-RCA sera which reacted specifically with bovine RCA in double agar gel diffusion and immunoelectrophoretic tests, but failed to cross-react with bovine erythrocyte CA. The localization of RCA was identified in histological sections and isolated ruminal epithelial cell preparations by indirect immunofluorescence and immunoperoxidase tests as the basal, spinosum and granulosum layers of ruminal mucous epithelium.
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References
Aafjes JH (1967) Carbonic anhydrase in the wall of the forestomachs of cows. Br Vet J 123:252–255
Armstrong JMcD, Myers DV, Verpoorte JA, Edsall JT (1966) Purification and properties of human erythrocyte carbonic anhydrase. J Biol Chem 241:5137–5149
Auton AJ, Barragry JM, Carter ND, Morris DV, Cohen RD (1976) Rapid diagnosis of thyroid disease using carbonic-anhydrase immunoassay. Lancet 2:1385–1387
Blake NM (1978) Genetic variants of carbonic anhydrase in the Asian-Pacific area. Ann Human Biol 5:557–568
Burns I, Hambridge M, Taylor CR (1974) Intracellular immunglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates. J Clin Pathol 27:548–557
Carter MJ (1971) The carbonic anhydrase in the rumen epithelial tissue of the ox. Biochim Biophys Acta 235:222–236
Carter N, Auton J (1975) Characterisation of carbonic anhydrase from tissue of the cat. Biochim Biophys Acta 410:220–228
Furth AJ (1968) Purification and properties of horse erythrocyte carbonic anhydrases. J Biol Chem 243:4832–4841
Gálfi P, Neogrády S, Kutas F (1981) Culture of epithelial cells from bovine ruminal mucosa. Vet Res Commun 4:295–300
Kumpulainen T (1979) Immunohistochemical localization of human carbonic anhydrase isoenzyme C. Histochemistry 62:271–280
Kumpulainen T, Korhonen LK (1978) Immunohistochemical demonstration of carbonic anhydrase. Histochemistry 58:183–192
Kutas F, Gálfi P, Neogrády S (1980) Study on the metabolism of epithelial cells isolated from rumen mucosa of cattle. XXVIII. International Congress of Physiological Sciences, Budapest. Hungary
Laurent G, Venot-Giraud N, Marriq C, Limozin N, Garcon D, Filippi D (1977) Selective tissue distribution of carbonic anhydrase isoenzymes in the ox. Biochim Biophys Acta 481: 222–226
Lauwers H (1973) Morfologische bijdrage tot de kennis van het resorberend vermogen van rundervoormagen. Communications the Faculty of Veterinary Medicine. State University Gent 17 1–2:75–111
Lindskog S (1960) Purification and properties of bovine erythrocyte carbonic anhydrase. Biochim Biophys Acta 39:218–226
Lowry OH, Rosebrough NJ, Farr AZ, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mallet B, Gulian JM, Sciaky M, Laurent G, Charrel M (1979) Formes molaculaires multiples de l'anhydrase carbonique erythrocytaire ovine. Biochim Biophys Acta 576:290–304
Morris BJ, Iwamoto HS, Reid IA (1979) Localisation of angiotensinogen in rat liver by immunocytochemistry. Endocrinology 105:796–800
Ormian M, Dola L (1979) Polymorphism of carbonic anhydrase in red cells of the polish cattle breeds. Genet Pol 20:247–255
Ouchterlory Ö (1968) Handbook of immunodiffusion and immunoelektrophoresis. Ann Arbor Science Publication, Ann Arbor
Register AM, Koester MK, Noltmann EA (1978) Discovery of carbonic anhydrase in rabbit skeletal muscle and evidence for its identity with “basic muscle protein”. J Biol Chem 253:4143–4152
Schulz H (1962a) Mikroskopisch-anatomischer Bau und Enzymbestand der Pansenzotten von Giraffa camelopardalis (L.) (Ein histochemischer Beitrag zur Physiologie der Ruminantier-Vormägen). Zool Anz 169:331–347
Schulz H (1962b) Histo-enzymologische Beobachtungen an den Pansenzotten des Rindes. Zool Anz 169:433–439
Schulz H (1963) Histochemische Untersuchungen an den Pansenzotten eines Hausyaks, Bos mutus f. grunniens L. Zool Anz 170:306–310
Smith I, Lightstone PJ, Perry JD (1968) Separation of human alkaline phosphatases by elektrophoresis on acrylamide disc gels. Clin Chim Acta 19:499–505
Spicer SS, Stoward PJ, Tashian RE (1979) The immunohistolocalisation of carbonic anhydrase in rodent tissue. J Histochem Cytochem 27:820–831
Stevens CE (1970) Fatty acid transport through the rumen epithelium. In: Phillipson AT (ed) Physiology of digestion and metabolism in the ruminant. Oriel, Newcastle, pp 101–112
Streefkerk JC (1972) Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxidase following methanol. J Histochem Cytochem 20:829–831
Wilbur K, Anderson NG (1948) Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem 176:147–154
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Gálfi, P., Kutas, F. & Neogrády, S. Immunohistochemical detection of bovine ruminal carbonic anhydrase isoenzyme. Histochemistry 74, 577–584 (1982). https://doi.org/10.1007/BF00496672
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DOI: https://doi.org/10.1007/BF00496672