Skip to main content
SpringerLink
Log in

Mucosubstance histochemistry of Brunner's glands, pyloric glands and duodenal goblet cells in the ferret

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

The mucosubstance of Brunner's glands, pyloric glands and duodenal goblet cells were studied using the various histochemical methods.

The secretions of both Brunner's and pyloric glands were similar in their histochemical reactions. They contained neutral mucosubstances as in these glands in man.

The duodenal goblet cells showed variations in their histochemical characters. (i) The secretions of most of the deep cells and the majority of superficial cells contained sialidase-labile and sialidase-resistant sialomucins. (ii) There were a few superficial and occasional deep cells, the secretions of which contained sulphated mucosubstances. (iii) There were some goblet cells, more in the villi than in the crypts, the secretions of which contained a mixture of sialomycins and a sulphated mucin. The sialomucin was mostly sialidase-labile and partly sialidase-resistant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abul-Haj, S.K., Rhinert, J.F.: Fuchsin-aldehyde staining of sulphate mucopolysaccharides and related substances. J. Natl. Cancer Inst. 13, 232 (1952–53)

    Google Scholar 

  • Allen, M.G.: The effect of acid hydrolysis on sialomucins at selected epithelial sites. Thesis for Fellowship of the Institute of Medical and Laboratory Technology (1970)

  • Belanger, L.F.: Autoradiographic visualisation of S35 incorporation and turnover by the mucous glands of the gastrointestinal tract and other soft tissues of rat and hamster. Anat. Rec. 118, 755–771 (1954)

    Google Scholar 

  • Belanger, L.F.: Comparisons between different histochemical and histophysical techniques as applied to mucus-secreting cells. Ann. N.Y. Acad. Sci. 106, 364–378 (1963)

    Google Scholar 

  • Berger, C., Pizzolato, P.: The staining of Brunner's gland and other neutral mucins by carmine, haematoxylin and orcein in alkaline solutions. Stain Technol. 50(6), 383–386 (1975)

    Google Scholar 

  • Bignardi, C.: Cellule mucose e cellule mucoidi I. Contributo critico e tecnico ale'impostazione di alcuni problemi generali. Arch. Ital. Anat. Embriol. 42, 389–409 (1939)

    Google Scholar 

  • Bignardi, C.: Celluli mucose e celluli mucoidi. VI Uteriori ricerche sui caratteri di colorabilita delle cellule mucoidi dopo cromizzazione. Atti Soc. Ital. Sci. Nat. 89, 69–83 (1940)

    Google Scholar 

  • Bignardi, C., Aureli, G., Balduini, C., Castellani, A.A.: Sulfo-sialo-polysaccharide-peptide from dog submaxillary gland. Biochem. Biophys. Res. Commun. 17, 310–312 (1964)

    Google Scholar 

  • Boerner-Patzelt, D.: Über die Verwandtschaft der Pylorusdrüsen und Duodenaldrüsen. Z. Mikr.-anat. Forsch. 51, 555–580 (1942)

    Google Scholar 

  • Burkl, W.: Untersuchungen über die Pylorus- und Duodenaldrüsen. Z. mikr.-anat. Forsch. 56, 327–414 (1950)

    Google Scholar 

  • Carvalho, A.D.V., Neto, R.S., Moreira, M.C.: Histoquimica de mucopolisacarides em gländulas duodenais (de Brünner) e cëlulas caliciformes de Dasypus novemcinctus. Arq. Cent. Estud. Fac. Odontol. Univ. Fed. Minas Gerais 7, 91–101 (1970)

    Google Scholar 

  • Carvalho, A.D.V., Neto, R.S., Ribeiro, Z.: Histochemistry of mucopolysaccharides in the gastric epithelium of the pyloric region in the Dasypus novemcinctus. Rev. Bras. Pes qui. Med. Biol. 6 (3–4), 181–186 (1973)

    Google Scholar 

  • Casoni, R.: Ricerche istochimiche sul secreto mucoide dell'epitelio gastrico del coniglio in varie condizioni sperimentali. Atti. Soc. Ital. Sci. Vet. 6, 1–4 (1952)

    Google Scholar 

  • Cochrane, W., Davies, D.V., Palfrey, A.J., Stockwell, R.A.: The histochemistry and electron microscopy of Brunner's glands in the guineapig. J. Anat. 98, 1–10 (1964)

    Google Scholar 

  • Cook, H.C.: A histochemical characterisation of malignant tumour mucins as a possible aid in the identification of metastatic deposits. Med. Lab. Technol. 30, 217–224 (1973)

    Google Scholar 

  • Curran, R.C., Kennedy, J.S.: The distribution of the sulphated mucopoly-saccharides in the mouse. J. Pathol. Bacteriol. 70, 449–457 (1955)

    Google Scholar 

  • Florey, H.W., Harding, H.E.: Further observations on the secretions of Brunner's glands. J. Pathol. Bacteriol. 39, 255–276 (1934)

    Google Scholar 

  • Franks, L.M., O'Shea, J.D., Thomson, A.E.R.: Mucins in the prostate: a histochemical study in normal glands, latent, clinical and colloid cancers. Cancer 17, 983–999 (1964)

    Google Scholar 

  • Ganter, P., Marche, Cl.: Histochimie des mucines gastrointestinales de l'homme. Ann. Anat. Pathol. (Paris) 15, 321–346 (1970)

    Google Scholar 

  • Gerard, A., Lev, R., Glass, G.B.J.: Histochemical study of the mucosubstances in the canine stomach. I. The resting mucosa. Am. J. Dig. Dis. 12, 891–912 (1967)

    Google Scholar 

  • Gomori, G.: Aldehyde fuchsin: a new stain for elastic tissue. Am. J. Clin. Pathol. 20, 665 (1950)

    Google Scholar 

  • Hale, C.W.: Histochemical demonstration of acid mucopolysaccharides in animal tissues. Nature 157, 802 (1946)

    Google Scholar 

  • Jacob, S., Poddar, S.: The histochemistry of mucosubstances in ferret salivary glands. Acta Histochem. (Jena) 61, 142–154 (1978)

    Google Scholar 

  • Jennings, M.A., Florey, H.W.: Autoradiographic observations on the mucous cells of the stomach and intestine. Q. J. Exp. Physiol. 41, 131–152 (1956)

    Google Scholar 

  • Jervis, H.R., Sheahan, D.G., Sprinz, H.: Acute duodenal ulcerations in the guineapig due to fasting. Delineation of experimental model. Lab. Invest. 28, 501–512 (1973)

    Google Scholar 

  • Kent, P.W., Marsden, J.O.: A sulphated sialoprotein from sheep colonic mucin. Biochem. J. 87, 38P-39P (1963)

    Google Scholar 

  • Krause, W.J.: Brunner's glands of the echidna. Anat. Rec. 167, 473–488 (1970)

    Google Scholar 

  • Krause, W.J.: Brunner's glands of the duckbilled platypus (Ornithorhynchus anatinus). Am. J. Anat. 132, 147–166 (1971)

    Google Scholar 

  • Krause, W.J.: Morphological and histochemical features of the duodenal glands in six marsupial species. J. Morphol. 140, 321–342 (1973)

    Google Scholar 

  • Krause, W.J., Leeson, C.R.: Studies of Brunner's glands in the opossum. I. Adult morphology. Am. J. Anat. 126, 255–274 (1969)

    Google Scholar 

  • Lamb, D., Reid, L.: Histochemical types of acidic glycoprotein produced by mucous cells of the tracheo-bronchial glands in man. J. Pathol. 98, 213–229 (1969)

    Google Scholar 

  • Lev, R., Spicer, S.S.: Specific staining of sulphate groups with alcian blue at low pH. J. Histochem. Cytochem. 12, 309 (1964)

    Google Scholar 

  • Lillie, R.D.: Studies on the histochemistry of normal and pathological mucins in man and in laboratory animals. Bull. Int. Assoc. Med. Mus. 29, 1–53 (1949)

    Google Scholar 

  • McManus, J.F.A.: Histochemical demonstration of mucin after periodic acid. Nature 158, 202 (1946)

    Google Scholar 

  • McManus, J.F.A., Mowry, R.: Staining methods, histological and histochemical. New York: Harper & Row 1960

    Google Scholar 

  • Miraglia, T., Ledoux, L., Castelo Branco, A.L.: Histological and histochemical data on the intestinal tract of the marmoset (Calliturix jacchus). Acta Anat. 68, 459–472 (1967)

    Google Scholar 

  • Mowry, R.W.: Alcian blue techniques for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem. 4, 407 (1956)

    Google Scholar 

  • Mowry, R.W.: The special value of methods that colour both acidic and vicinyl hydroxyl groups in the histochemical study of mucins, with revised directions for the colloidal iron stain, the use of alcian blue 8Gx and their combination with the periodic acid Schiff reaction. Ann. NY Acad. Sci. 106, 402–423 (1963)

    Google Scholar 

  • Murgatroyd, L.B.: Studies on histochemical demonstration of glycogen. Thesis for Fellowship of the Institute of Medical and Laboratory Technology (1969)

  • Obuoforibo, A.A.: Mucosubstances in Brunner's glands of the mouse. J. Anat. 119, 287–294 (1975)

    Google Scholar 

  • Odour-Okelo, D.: Histochemistry of the duodenal glands of the cat and horse. Acta Anat. 94, 449–456 (1976)

    Google Scholar 

  • Pearse, A.G.E.: Histochemistry, theoretical and applied. 3rd ed. Vol. 1. London: Churchill 1969

    Google Scholar 

  • Poddar, S., Murgatroyd, L.: Morphological and histological study of the gastrointestinal tract of the ferret. Acta Anat. 96, 321–334 (1976)

    Google Scholar 

  • Scott, J.E., Dorling, J.: Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions. Histochemie 5, 221–233 (1965)

    Google Scholar 

  • Sheahan, D.G., Jervis, H.R.: Comparative histochemistry of gastrointestinal mucosubstances. Am. J. Anat. 146, 103–132 (1976)

    Google Scholar 

  • Sheahan, D.G., Jervis, H.R., Takeuchi, A., Sprinz, J.: The effect of staphylococcal enterotoxin on the epithelial mucosubstances of the small intestine of Rhesus monkey. Am. J. Pathol. 60, 1–18 (1970)

    Google Scholar 

  • Spicer, S.S.: A correlative study of the histochemical properties of rodent acid mucopolysaccharides. J. Histochem. Cytochem. 8, 18–35 (1960)

    Google Scholar 

  • Spicer, S.S.: The use of cationic reagents in histochemical differentiation of mucopolysaccharides. Am. J. Clin. Pathol. 36, 393–407 (1961)

    Google Scholar 

  • Spicer, S.S.: Histochemical differentiation of mammalian mucopolysaccharides. Ann. NY Acad. Sci. 106, 379–388 (1963)

    Google Scholar 

  • Spicer, S.S.: Diamine methods for differentiating mucopolysaccharides histochemically. J. Histochem. Cytochem. 13, 211 (1965)

    Google Scholar 

  • Spicer, S.S., Lillie, R.D.: Saponification as a means of selectively revising the methylation blockade of tissue basophilia. J. Histochem. Cytochem. 7, 123–125 (1959)

    Google Scholar 

  • Spicer, S.S., Meyer, D.B.: Histochemical differentiation of acid mucopolysaccharides by means of combined aldehyde fuchsin-alcian blue staining. Am. J. Clin. Pathol. 33, 453–460 (1960)

    Google Scholar 

  • Spicer, S.S., Leppi, T.J., Stoward, P.J.: Suggestions for a histochemical terminology of carbohydraterich tissue components. J. Histochem. Cytochem. 13, 599–603 (1965)

    Google Scholar 

  • Spicer, S.S., Sun, D.C.H.: Carbohydrate histochemistry of gastric epithelial secretion in dog. Ann. NY Acad. Sci. 140, 762–783 (1967)

    Google Scholar 

  • Williams, G., Jackson, D.S.: Two organic fixatives for acid mucopolysaccharides. Stain Technol. 31, 189–191 (1956)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of the West Indies, Mona, Kingston 7, Jamaica, W.I.

    S. Poddar

  2. Department of Human Biology and Anatomy, University of Sheffield, S10 2TN, Sheffield, England

    S. Jacob

Authors
  1. S. Poddar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Jacob
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Poddar, S., Jacob, S. Mucosubstance histochemistry of Brunner's glands, pyloric glands and duodenal goblet cells in the ferret. Histochemistry 65, 67–81 (1979). https://doi.org/10.1007/BF00496687

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00496687

Keywords

Search

Navigation