Summary
The reaction mechanism of “Koelle's thiocholine method” and “Karnovsky's ferricyanide method” for the localization of acetylcholinesterase activity have been studied. Some modifications for these two methods have been proposed.
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Tsuji, S. On the chemical basis of thiocholine methods for demonstration of acetylcholinesterase activities. Histochemistry 42, 99–110 (1974). https://doi.org/10.1007/BF00498482
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DOI: https://doi.org/10.1007/BF00498482