Summary
The authors studied the distribution of lipids in the submaxillary and sublingual salivary gland of the guinea pig. By means of histochemical and physico-chemical methods the chemical composition of these lipids is caracterized. In the various secreting units, the results show mainly lipoproteins (phospho- and sphingolipoproteins). The lipoproteins are present under various structures crystallin double-refractil spheroid, granular mono-refractil, diffuse mono-refractil. The lipidic component is not identical in all these lipoproteins. The neutral lipids and the fatty acids are rare. Glycolipids are presents in various secreting units of the serous gland.
Résumé
Les auteurs ont étudié la distribution des corps gras dans les glandes sous-maxillaires et sublinguales de cobaye. Avec des méthodes physico-chimiques et histochimiques, ceux-ci ont été caractérisés du point de vue chimique. Les résultats ont mis en évidence principalement des lipoprotéines (phospho- et sphingolipoprotéines) distribuées différemment dans les unités sécrétantes. Les lipoprotéines se présentent sous plusieurs formes: cristalline biréfringente, sphéroïde, granulaire monoréfringente, diffuse monoréfringente. Le composant lipidique n'est pas identique pour toutes les lipoprotéines. Les lipides neutres et les acides gras sont rares. Des glycolipides peuvent être présents au niveau des différentes unités sécrétantes de la glande séreuse.
Similar content being viewed by others
Bibliographie
Adams, C. W. M.: A histochemical method for the simultaneous demonstration of normal and degenerating myelin. J. Path. Bact. 77, 648–650 (1959)
Adams, C. W. M.: Neurohistochemistry. Histochemistry of lipids, p. 7–66. London: Elsevier Publishing Company 1965
Dunnigan, M. G.: The use of nile blue sulphate in the histochemical identification of phospholipides. Stain Technol. 43, 249–256 (1968)
Flon, H., Gerstuer, R.: Salivary glands of the hamster. I. The submandibular gland: a histochemical study after preservation with various fixatives. Acta histochem. (Jena) 31, 234–253 (1968)
Ganter, P., Jolles, G.: Histochimie normale et pathologique, vol. 1, vol. 2. Paris: Gauthier-Villars 1969, 1970
Hollmann, K. H., Verley, J. M.: La glande sous-maxillaire de la souris et du rat. Etude au microscope électronique. Z. Zellforsch. 68, 363–388 (1965)
Leblond, C. P.: Distribution of periodic acid-reactive carbohydrates in the adult rat. Amer. J. Anat. 86, 1–49 (1950)
Lison, L.: Histochimie et cytochimie animales. Principes et méthodes, vol. 2. Paris: Gauthier-Villars 1960
Menschik, Z.: Nil blue histochemical method for phospholipids. Stain Technol. 28, 13–18 (1953)
Pearse, A. G. E.: Histochemistry. Theoretical and applied, vol. 1, vol. 2. London: J. A. Churchill Ltd. 1968, 1972
Policard, A., Bessis, M., Locquin, M.: Traité de microscopie. Instruments et techniques, p. 490–498 Paris: Masson & Cie 1957
Quintarelli, G.: Studies of sialic acid-containing mucins in bovine submaxillary and rat sublingual glands. J. Histochem. Cytochem. 9, 176–183 (1961)
Quintarelli, G.: Histochemical identification of salivary mucins. Ann. N.Y. Acad. Sci. 106, 339–363 (1963)
Ravetto, C., Galluzzo, F., Siervo, R.: On the presence of a ganglioside in bovine submaxillary gland. J. Histochem. Cytochem. 12, 791–792 (1964)
Scheuner, G., Hutschenseiter J.: Polarisationsmikroskopie in der Histophysik, S. 153–157. Leipzig: VEB Georg Thieme 1972
Schulz, H., De Paola, D.: Delta-cytomembranen und lamelläre Cytosomen, Ultrastruktur, Histochemie und ihre Beziehungen zur Schleimsekretion. Z. Zellforsch. 49, 125–141 (1958)
Scott, B. L., Pease, D. C.: Electron microscopy of the salivary and lacrimal glands of the rat. Amer. J. Anat. 104, 115–161 (1959)
Scott, B. L., Pease, D. C.: Electron microscopy of induced changes in the the salivary gland of the rat. In: salivary glands and their secretions, publié par L. M. Sreebny et J. Meyer, p. 13–44. International series of monographs on oral biology, London: Pergamon Press 1964
Shackleford, J. M.: Histochemical comparison of mucous secretions in rodent, carnivore, ungulate and primate major salivary glands. Ann. N.Y. Acad. Sci. 106, 572–582 (1963)
Shackleford, J. M., Klapper, C. E.: Structure and carboydrate histochemistry of mammalian salivary glands. Amer. J. Anat., 3, 25–48 (1962)
Simson, J. A. V., Hall, B. J., Spicer, S. S.: Histochemical evidence for lipoïd material in secretory granules of rat salivary glands. Histochem. J. 5, 239–254 (1973)
Stormont, D. L.: The salivary glands. In: Special cytology, publié par E. V. Cowdry, vol. I, p. 153–196. New York: Hoeber 1932
Warren, L., Spicer, S. S.: Biochemical and histochemical identification of sialic acid containing mucins of rodent vagina and salivary glands. J. Histochem. Cytochem. 9, 400–408 (1961)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gotzos, V., Musy, J.P. Etude histochimique des lipides des glandes sous-maxillaires et sublinguales du cobaye. Histochemistry 41, 145–160 (1974). https://doi.org/10.1007/BF00499130
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00499130